Compés Esther, Hernández Elena, Quirce Santiago, Palomares Oscar, Rodríguez Rosalía, Cuesta Javier, Sastre Joaquín, Villalba Mayte
Fundación Jiménez Díaz, Allergy Department, Universidad Autónoma de Madrid, Spain.
Ann Allergy Asthma Immunol. 2006 Apr;96(4):586-92. doi: 10.1016/S1081-1206(10)63554-5.
The allergenicity of the ornamental tree Robinia pseudoacacia, or black locust, is unknown.
To evaluate the prevalence of sensitization to R. pseudoacacia pollen, its possible allergenic cross-reactivity with other common pollens, and the potential implication of pollen panallergens (profilin, polcalcin, and 1,3-beta-glucanase) as a cause of sensitization to R. pseudoacacia pollen.
Skin prick testing with R. pseudoacacia pollen was performed in 149 patients with pollinosis. Nasal challenge with R. pseudoacacia pollen was performed in 10 patients. The prevalence of sensitization to the recombinant forms of profilin (rChe a 2), polcalcin (rChe a 3), and the N-terminal of the 1,3-beta-glucanase (rNtD of Ole e 9) was investigated. Immunoblotting, enzyme-linked immunosorbent assay, and competitive inhibition assays were performed with R. pseudoacacia pollen and recombinant pollen allergens.
Sixty-four patients (43%) had positive skin prick test reactions to R. pseudoacacia pollen. Nasal challenge results were positive in 5 sensitized patients and negative in 4 controls and 1 sensitized patient. The allergenic profile of R. pseudoacacia pollen comprises at least the panallergen profilin, a calcium-binding protein, and a 1,3-beta-glucanase. The prevalence of sensitization to rChe a 2, rChe a 3, and rNtD of Ole e 9 was 60%, 33%, and 87%, respectively, among patients sensitized to R. pseudoacacia pollen. Binding of IgE to R. pseudoacacia extract was completely inhibited by Robinia, Chenopodium, Olea, Cupressus, and Lolium extracts.
The high prevalence of R. pseudoacacia pollen sensitization in patients with pollinosis is likely to be due to cross-sensitization to panallergens (profilin, polcalcin, and 1,3-beta-glucanase) from other common pollens. This phenomenon may lead to a diagnosis of "allergy mirages."
观赏树刺槐（Robinia pseudoacacia），即洋槐，的致敏性尚不清楚。
评估对刺槐花粉致敏的患病率、其与其他常见花粉可能存在的交叉致敏性，以及花粉泛变应原（肌动蛋白结合蛋白、钙结合蛋白和1,3-β-葡聚糖酶）作为刺槐花粉致敏原因的潜在影响。
对149例花粉症患者进行刺槐花粉皮肤点刺试验。对10例患者进行刺槐花粉鼻激发试验。研究对重组形式的肌动蛋白结合蛋白（rChe a 2）、钙结合蛋白（rChe a 3）和1,3-β-葡聚糖酶N端（rNtD of Ole e 9）致敏的患病率。用刺槐花粉和重组花粉变应原进行免疫印迹、酶联免疫吸附测定和竞争性抑制试验。
64例患者（43%）对刺槐花粉皮肤点刺试验反应阳性。5例致敏患者鼻激发试验结果为阳性，4例对照患者和1例致敏患者为阴性。刺槐花粉的变应原谱至少包括泛变应原肌动蛋白结合蛋白、一种钙结合蛋白和一种1,3-β-葡聚糖酶。在对刺槐花粉致敏的患者中，对rChe a 2、rChe a 3和rNtD of Ole e 9致敏的患病率分别为60%、33%和87%。刺槐提取物、藜草提取物、油橄榄提取物、柏木提取物和黑麦草提取物可完全抑制IgE与刺槐提取物的结合。
花粉症患者中刺槐花粉致敏的高患病率可能是由于对来自其他常见花粉的泛变应原（肌动蛋白结合蛋白、钙结合蛋白和1,3-β-葡聚糖酶）的交叉致敏。这种现象可能导致“过敏幻影”的诊断。
Zotero 插件