Barber D, de la Torre F, Feo F, Florido F, Guardia P, Moreno C, Quiralte J, Lombardero M, Villalba M, Salcedo G, Rodríguez R
Departamento de I+D, ALK-Abelló, Madrid, Spain.
Allergy. 2008 Nov;63(11):1550-8. doi: 10.1111/j.1398-9995.2008.01807.x.
Allergy diagnosis in patients exposed to multiple pollen species is complex and misdiagnosis is often a cause for unsuccessful specific immunotherapy.
We studied the sensitization profile of individual allergens (major, minor and pan-allergens) in pollen-sensitized patients in a region with high exposure to olive pollen by investigating the influence of minor allergens on allergic disease and the association between pan- and minor allergen sensitizations.
A panel of 13 purified allergens, which included the most relevant allergens in the area, as well as minor olive allergens and pan-allergens, were screened using a high-capacity technology (ADVIA-Centaur) in 891 patients.
Olive allergy as measured by specific IgE to Ole e 1 was the leading pollinosis in the area. The minor olive allergens Ole e 7 and Ole e 9 were markers of more severe allergic illness. Profilin sensitization was associated mainly with grass allergy, the second most prevalent pollinosis. Salsola kali pollen allergy was the third most common cause of pollinosis in the area. The prevalence of sensitization to the peach allergen Pru p 3, a nonspecific lipid-transfer protein, was notable.
Epidemiological analysis by component-resolved diagnosis is a new method, which elucidates the interaction between allergen exposure gradient and patient sensitization. High exposure leads to differential sensitization profiles some of which are associated with more severe allergic conditions. Profilin sensitization, related mainly to grass pollinosis, was a marker of more severe grass pollen sensitization.
暴露于多种花粉种类的患者的过敏诊断较为复杂，误诊常常是特异性免疫治疗失败的原因。
通过研究次要过敏原对过敏性疾病的影响以及泛过敏原与次要过敏原致敏之间的关联，我们对花粉致敏患者中个体过敏原（主要、次要和泛过敏原）的致敏情况进行了研究，该地区橄榄花粉暴露量较高。
使用高容量技术（ADVIA-Centaur）对891例患者进行了一组13种纯化过敏原的筛查，其中包括该地区最相关的过敏原，以及次要橄榄过敏原和泛过敏原。
通过对油橄榄花粉主要过敏原Ole e 1的特异性IgE检测所测得的橄榄过敏是该地区主要的花粉症。次要橄榄过敏原Ole e 7和Ole e 9是更严重过敏性疾病的标志物。肌动蛋白结合蛋白致敏主要与禾本科花粉过敏相关，禾本科花粉过敏是第二常见的花粉症。猪毛菜花粉过敏是该地区第三常见的花粉症病因。对桃过敏原Pru p 3（一种非特异性脂质转移蛋白）的致敏率值得注意。
通过组分分辨诊断进行的流行病学分析是一种新方法，它阐明了过敏原暴露梯度与患者致敏之间的相互作用。高暴露导致不同的致敏情况，其中一些与更严重的过敏状况相关。主要与禾本科花粉症相关的肌动蛋白结合蛋白致敏是更严重的禾本科花粉致敏的标志物。
