Sun Xing-hui, Wu Jun, Yi Shao-xuan, Yang Shun-liang, Wu We-zhen, Tan Jian-ming
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical Unversity, Chongqing 400038, PR China.
Zhonghua Shao Shang Za Zhi. 2006 Feb;22(1):46-9.
To explore the immunosuppressive effect of local transfection of Molluscum contagiosum virus 148 (MC148) gene to allogenous skin graft against rejection.
MC148 gene was cloned from molluscum contagiosum virus (MCV), and was employed to construct recombinant adenovirus vector (Ad-MC148). The recombinant Ad-MC148 was then locally transfected into a part of the tail skin of eight Lewis rats, which served as skin donors for grafting. The wounds (1 cm x 1 cm) were produced on the tails of 16 Wistar rats, and they were then randomly divided into control (C, n=8, with grafting of skin from donor rats without transfection), and transfection (T, n=8, with grafting of skin from donor rats with transfection of the recombinant Ad-MC148) groups. The expression of MC148 mRNA gene in T group was detected on 6 post operation hour( POH) and 2, 3, 7 and 10 post operation day (POD), and the results were expressed by the ratio of absorption value (A) between MC148 gene and beta-actin. The survival time of skin grafts in both groups was compared. Gross examination of grafted skin was carried out from 7 POD on in both groups, and the pathomorphological changes were examined in both groups on 7 POD.
The MC148 gene expression in rat skin of T group could be identified in 6 POH, and it reached the peak on 3 POD (A(MC148 mRNA) / A(beta_actin) = 0.86), and then subsided thereafter, but it maintained for 10 days. The survival time of the grafts in T group was (15.0 +/- 2.0) days, and it was significantly longer than that in C group (8.5 +/- 3.4) days, (P < 0.01). Gross and microscopic examination showed that the tail skin of T group appeared ruddy on 7 POD, with little leukocytic infiltration in subcutaneous tissue; it began to turn black after 12 to 20 PODs. On the other hand, the tail skin of C group began to turn black and to shed off on 7 POD, with evident leukocytic infiltration in subcutaneous tissue and dermis.
Local transfection of MC148 gene may promote immunosuppression by inhibiting leukocytic infiltration after allogenous skin transplantation.
探讨局部转染传染性软疣病毒148（MC148）基因对同种异体皮肤移植排斥反应的免疫抑制作用。
从传染性软疣病毒（MCV）中克隆MC148基因，构建重组腺病毒载体（Ad-MC148）。将重组Ad-MC148局部转染8只Lewis大鼠的部分尾部皮肤，作为供皮用于移植。在16只Wistar大鼠的尾部制作伤口（1 cm×1 cm），然后将其随机分为对照组（C组，n = 8，移植未转染的供体大鼠皮肤）和转染组（T组，n = 8，移植转染重组Ad-MC148的供体大鼠皮肤）。在术后6小时（POH）以及术后第2、3、7和10天（POD）检测T组中MC148 mRNA基因的表达，结果以MC148基因与β-肌动蛋白的吸光度比值（A）表示。比较两组皮肤移植的存活时间。两组均从术后第7天开始对移植皮肤进行大体检查，并在术后第7天检查病理形态学变化。
T组大鼠皮肤中MC148基因在术后6小时即可检测到表达，在术后第3天达到峰值（A（MC148 mRNA）/A（β-肌动蛋白） = 0.86），随后下降，但持续10天。T组移植皮片的存活时间为（15.0±2.0）天，显著长于C组的（8.5±3.4）天，（P < 0.01）。大体和显微镜检查显示，T组尾部皮肤在术后第7天呈红润色，皮下组织白细胞浸润较少；在术后12至20天开始变黑。另一方面，C组尾部皮肤在术后第7天开始变黑并脱落，皮下组织和真皮层有明显的白细胞浸润。
局部转染MC148基因可能通过抑制同种异体皮肤移植后白细胞浸润来促进免疫抑制。
