Gospodarowicz D, Handley H H
Endocrinology. 1975 Jul;97(1):102-7. doi: 10.1210/endo-97-1-102.
The effect of Fibroblast Growth Factor (FGF) on the initiation of DNA synthesis and on the rate of division of the Y1 adrenal cell line has been investigated. In sparse populations of Y1 cells (4 times 10- minus 3 cells/cm2) maintained in 0.2 percent calf serum, FGF was able to initiate DNA synthesis to the same extent that an optimal concentration of serum could. Cells maintained in 0.2 percent calf serum sustained continuous growth when given 5 ng/ml of FGF daily. Cultures fixed and stained with crystal violet showed FGF colonies to be of equivalent size and quantity as those with serum alone. Insulin had no mitogenic activity of its own at concentrations as high as 500 ng/ml nor did it have any potentiating effect on the mitogenic activity of FGF. Glucocorticoids (0.1 mug/ml to 1 mug/ml) inhibited (25 percent) the initiation of DNA synthesis as well as the rate of division induced by FGF. ACTH (0.75 IU/ml) was clearly inhibitory. Not only did it reduce the rate of division of cells in serum but it also reduced the rate of DNA synthesis and inhibited division in the presence of FGF.
研究了成纤维细胞生长因子（FGF）对Y1肾上腺细胞系DNA合成起始及分裂速率的影响。在0.2%小牛血清中培养的稀疏Y1细胞群体（4×10⁻³个细胞/cm²）中，FGF启动DNA合成的程度与最佳浓度血清相同。每天给予5 ng/ml FGF时，在0.2%小牛血清中培养的细胞能持续生长。用结晶紫固定和染色的培养物显示，FGF形成的集落大小和数量与仅含血清的集落相当。胰岛素在高达500 ng/ml的浓度下自身无促有丝分裂活性，对FGF的促有丝分裂活性也无增强作用。糖皮质激素（0.1 μg/ml至1 μg/ml）抑制（25%）DNA合成的起始以及FGF诱导的分裂速率。促肾上腺皮质激素（0.75 IU/ml）有明显抑制作用。它不仅降低血清中细胞的分裂速率，还降低DNA合成速率，并在有FGF存在时抑制分裂。
