成纤维细胞生长因子对人及小鼠成纤维细胞早期传代培养物的促有丝分裂作用。
Mitogenic effect of fibroblast growth factor on early passage cultures of human and murine fibroblasts.
作者信息
Gospodarowicz D, Moran J S
出版信息
J Cell Biol. 1975 Aug;66(2):451-7. doi: 10.1083/jcb.66.2.451.
Abstract
Fibroblast growth factor (FGF), a polypeptide that has been shown to stimulate division in 3T3 cells, was tested for mitogenic effects on diploid, early-passage cells from human and murine sources. The quantitative assay of [3H]thymidine incorporation into acid-insoluble material showed that FGF at low concentrations (10 minus 9 M) was more effective than additional serum for provoking the initiation of DNA synthesis in human foreskin fibroblasts or mouse fibroblasts maintained in 5 or 10% serum, respectively. The growth of the human fibroblasts was twice as fast in the presence of FGF plus 10% calf serum as it was in the presence of 10% calf serum or 20% fetal calf serum alone. The addition of FGF to primary cultures of mouse fibroblasts in 0.4% serum resulted in a twofold increase in cell number compared to controls. In contrast to results obtained with 3T3 cells, neither insulin nor a glucocorticoid potentiated the effects of FGF on either human or mouse cells.
摘要
成纤维细胞生长因子（FGF）是一种已被证明能刺激3T3细胞分裂的多肽，对来自人和小鼠的二倍体、早期传代细胞的促有丝分裂作用进行了测试。将[3H]胸腺嘧啶核苷掺入酸不溶性物质的定量分析表明，低浓度（10的负9次方摩尔）的FGF比额外添加血清更有效地激发分别在5%或10%血清中培养的人包皮成纤维细胞或小鼠成纤维细胞中DNA合成的起始。在添加FGF加10%小牛血清的情况下，人成纤维细胞的生长速度是仅添加10%小牛血清或20%胎牛血清时的两倍。在0.4%血清中向小鼠成纤维细胞原代培养物中添加FGF，与对照组相比，细胞数量增加了两倍。与在3T3细胞中获得的结果相反，胰岛素和糖皮质激素均不能增强FGF对人或小鼠细胞的作用。
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