Li Du-juan, Xu Zheng-shun, Zhang Zhao-hui, Huang Qing-yu
Department of Pathology, Henan University of Science and Technology, Luoyang, China.
Zhonghua Nan Ke Xue. 2006 Apr;12(4):318-22.
To observe the protective effects of vitamin E on the testicular injury by cyclophosphamide in mice, and the correlative mechanism.
Fifty sexually mature male mice were randomly divided into five groups: the cyclophosphamide group (the CP group), the low-dose vitamin E group (the low-dose group), the middle-dose vitamin E group (the middle-dose group), the high-dose vitamin E group (the high-dose group), the matched control group (the control group). The first four groups were given cyclophosphamide by gavage at a dose of 5 mg/(kg x d). The low-dose group, the middle-dose group and the high-dose group were given vitamin E by subcutaneous injection at doses of 30 mg/(kg x d), 50 mg/(kg x d) , 70 mg/(kg x d) after 4 h of cyclophosphamide treatment. The control group was gavaged with equivalent normal saline. The treatment period for all groups was 28 days. The level of plasma FSH, LH, T and the activity of testicular SOD, GSHPx, CAT and the level of testicular MDA were detected. The histological structure and the ultrastructure of the testis were examined by light microscope and electron microscope.
As compared with the CP group, the plasma FSH, LH, T level and the SOD, GSHPx, CAT activity in the middle-dose group and the high-dose group were higher (P< 0.05, P< 0.01), MDA level significantly lower(P<0.01). The histological structure and the ultrastructure of the testis were in the normal range.
Vitamin E has protective effects on the testicular injury by cyclophosphamide in mice. The possible mechanism of vitamin E may be its scavenging free radical and antioxidant effects, as well as it may have some stimulatory effects on gonadotrophin releasing of pituitary anterior lobe.
观察维生素E对环磷酰胺所致小鼠睾丸损伤的保护作用及其相关机制。
将50只性成熟雄性小鼠随机分为5组：环磷酰胺组（CP组）、维生素E低剂量组（低剂量组）、维生素E中剂量组（中剂量组）、维生素E高剂量组（高剂量组）、空白对照组（对照组）。前4组小鼠按5mg/(kg·d)剂量灌胃给予环磷酰胺。环磷酰胺处理4h后，低剂量组、中剂量组和高剂量组分别按30mg/(kg·d)、50mg/(kg·d)、70mg/(kg·d)剂量皮下注射维生素E。对照组给予等量生理盐水灌胃。所有组的处理周期均为28天。检测血浆卵泡刺激素（FSH）、黄体生成素（LH）、睾酮（T）水平以及睾丸超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHPx）、过氧化氢酶（CAT）活性和睾丸丙二醛（MDA）水平。用光镜和电镜观察睾丸的组织结构和超微结构。
与CP组相比，中剂量组和高剂量组血浆FSH、LH、T水平及SOD、GSHPx、CAT活性升高（P<0.05，P<0.01），MDA水平显著降低（P<0.01）。睾丸的组织结构和超微结构在正常范围内。
维生素E对环磷酰胺所致小鼠睾丸损伤具有保护作用。维生素E的可能作用机制可能是其清除自由基和抗氧化作用，以及对垂体前叶促性腺激素释放可能有一定的刺激作用。
