[己酮可可碱对大鼠睾丸扭转/扭转复位后精子发生的保护作用]

[Protective effect of pentoxifylline on spermatogenesis following testicular torsion/detorsion in rats].

作者信息

Liu Zi-ming, Zheng Xin-min, Yang Zhi-wei, Li Shi-wen

机构信息

Department of Urology, Zhongnan Hospital, Wuhan University, Hubei, China.

出版信息

Zhonghua Nan Ke Xue. 2006 Apr;12(4):323-5, 329.

PMID:16683564

Abstract

OBJECTIVE

To investigate the protective effect of pentoxifylline on spermatogenesis following testicular torsion/detorsion in rats.

METHODS

Healthy male Sprague-Dawley rats (n = 24) were divided into three groups randomly, each comprising 8 rats. In Group I, rats underwent a sham operation. In Group II and III, animals were submitted to unilateral 720 degrees testicular torsion, then detorsion in two hours. Infusion of isotonic saline and pentoxifylline into tail vein was initiated 15 minutes prior to relief of torsion in Group II and III respectively. Twenty four hours later, testes were examined for evidence of germ cell apoptosis by the flow cytometry and the level of total antioxidant capability (T-AOC) and malondialdehyde (MDA) by spectrophotometry.

RESULTS

Compared with that of group II, the number of apoptotic germ cell and the level of MDA decreased remarkably in Group III, but T-AOC increased significantly (P <0.01).

CONCLUSION

Pentoxifylline provided significant rescue of testicular function after acute experimental torsion.

摘要

目的

探讨己酮可可碱对大鼠睾丸扭转/复位后精子发生的保护作用。

方法

将24只健康雄性Sprague-Dawley大鼠随机分为三组，每组8只。第一组大鼠接受假手术。第二组和第三组动物接受单侧720度睾丸扭转，然后在两小时后复位。分别在第二组和第三组扭转解除前15分钟开始经尾静脉输注等渗盐水和己酮可可碱。24小时后，通过流式细胞术检测睾丸生殖细胞凋亡情况，通过分光光度法检测总抗氧化能力（T-AOC）和丙二醛（MDA）水平。