Liu Zi-ming, Zheng Xin-min, Yang Zhi-wei, Li Shi-wen
Department of Urology, Zhongnan Hospital, Wuhan University, Hubei, China.
Zhonghua Nan Ke Xue. 2006 Apr;12(4):323-5, 329.
To investigate the protective effect of pentoxifylline on spermatogenesis following testicular torsion/detorsion in rats.
Healthy male Sprague-Dawley rats (n = 24) were divided into three groups randomly, each comprising 8 rats. In Group I, rats underwent a sham operation. In Group II and III, animals were submitted to unilateral 720 degrees testicular torsion, then detorsion in two hours. Infusion of isotonic saline and pentoxifylline into tail vein was initiated 15 minutes prior to relief of torsion in Group II and III respectively. Twenty four hours later, testes were examined for evidence of germ cell apoptosis by the flow cytometry and the level of total antioxidant capability (T-AOC) and malondialdehyde (MDA) by spectrophotometry.
Compared with that of group II, the number of apoptotic germ cell and the level of MDA decreased remarkably in Group III, but T-AOC increased significantly (P <0.01).
Pentoxifylline provided significant rescue of testicular function after acute experimental torsion.
探讨己酮可可碱对大鼠睾丸扭转/复位后精子发生的保护作用。
将24只健康雄性Sprague-Dawley大鼠随机分为三组，每组8只。第一组大鼠接受假手术。第二组和第三组动物接受单侧720度睾丸扭转，然后在两小时后复位。分别在第二组和第三组扭转解除前15分钟开始经尾静脉输注等渗盐水和己酮可可碱。24小时后，通过流式细胞术检测睾丸生殖细胞凋亡情况，通过分光光度法检测总抗氧化能力（T-AOC）和丙二醛（MDA）水平。
与第二组相比，第三组凋亡生殖细胞数量和MDA水平显著降低，但T-AOC显著升高（P<0.01）。
己酮可可碱在急性实验性扭转后对睾丸功能有显著的挽救作用。
