Liu Zi-Ming, Zheng Xin-Min, Li Shi-Wen, Yang Zhi-Wei, Hu Li-Quan
Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, China.
Zhonghua Nan Ke Xue. 2003 Feb;9(1):40-2, 68.
To investigate the relationship between germ cell apoptosis and expression of Bcl-2 and Bax in experimental torsed/detorsed testes of the adult male rats.
Thirty healthy male Sprague-Dawley rats were divided into torsion group (n = 15) and control group (n = 15) randomly. Animals were submitted to unilateral 720 testicular torsion, then detorsion were done in two hours. Three days later, the evidence of germ cell apoptosis was detected by the TdT-mediated dUTP-biotin nick end labeling (TUNEL) technique. The expression of Bcl-2 and Bax were detected by immunohistochemical method.
In the torsed testes, the apoptosis index of germ cell and Bax expression significantly increased compared with that in the control group (P < 0.01) while Bcl-2 expression obviously decreased (P < 0.01). The apoptotic cells were mostly pechytene spermatocytes and round spermatides.
The germ cell apoptosis is highly associated with expression of Bcl-2 and Bax in experimental testicular torsion. Bcl-2/Bax plays an important role in germ cell apoptosis.
探讨成年雄性大鼠实验性睾丸扭转/复位后生殖细胞凋亡与Bcl-2和Bax表达之间的关系。
将30只健康雄性Sprague-Dawley大鼠随机分为扭转组（n = 15）和对照组（n = 15）。对动物进行单侧720°睾丸扭转，然后在两小时后进行复位。三天后，采用TdT介导的dUTP生物素缺口末端标记（TUNEL）技术检测生殖细胞凋亡情况。采用免疫组织化学方法检测Bcl-2和Bax的表达。
与对照组相比，扭转睾丸中生殖细胞凋亡指数和Bax表达显著增加（P < 0.01），而Bcl-2表达明显降低（P < 0.01）。凋亡细胞主要为粗线期精母细胞和圆形精子细胞。
在实验性睾丸扭转中，生殖细胞凋亡与Bcl-2和Bax的表达密切相关。Bcl-2/Bax在生殖细胞凋亡中起重要作用。
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