Zhang Hai-feng, Li Jian-zhong, Zhang Chun-ying
Department of Urology, the Second Hospital of Harbin Medical University, Heilongjiang, China.
Zhonghua Nan Ke Xue. 2006 Apr;12(4):349-51.
To study the impacts of positive antisperm antibody (AsAb) in seminal plasma on acrosomal enzyme activity, nitric oxide synthase (NOS) and superoxide dismutase (SOD) levels of spermatozoa.
Swatch from 40 infertile patients with positive AsAb in seminal plasma as experimental group, and 40 fertile men as control group. Acrosomal enzyme activity was detected by the BAEE/ADH unitive method, NOS was detected by the redoxreaction assay, and SOD level was measured xanthine oxidase method.
Compared with control group, acrosomal enzyme activity of spermatozoa of experimental group was significantly decreased (P <0.01), NOS activity was apparently increased (P < 0.01), and SOD level in seminal plasma was markedly decreased (P<0.01).
It may be possible that the positive AsAb in seminal plasma beget infertility through the changes of acrosomal enzyme of spermatozoa, SOD and NOS activities in seminal plasma.
研究精浆中抗精子抗体（AsAb）阳性对精子顶体酶活性、一氧化氮合酶（NOS）及超氧化物歧化酶（SOD）水平的影响。
选取40例精浆中AsAb阳性的不育患者作为实验组，40例生育男性作为对照组。采用BAEE/ADH联合法检测顶体酶活性，采用氧化还原反应法检测NOS，采用黄嘌呤氧化酶法测定SOD水平。
与对照组相比，实验组精子顶体酶活性显著降低（P<0.01），NOS活性明显升高（P<0.01），精浆中SOD水平显著降低（P<0.01）。
精浆中AsAb阳性可能通过精子顶体酶、精浆中SOD及NOS活性的改变导致不育。
