Grubor Nenad M, Liu Ying, Han Xinxin, Armstrong Daniel W, Jankowiak Ryszard
Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.
J Am Chem Soc. 2006 May 17;128(19):6409-13. doi: 10.1021/ja058424m.
The interaction of the highly cross-reactive anti-PAH monoclonal antibody with four diastereomeric benzo[a]pyrene tetrols (BPTs) is studied by means of fluorescence line-narrowing spectroscopy. It is shown that the interaction of enantiomers of cis-BPT and trans-BPT with the antibody involves different complex geometries. These spatially different ligand-protein interactions alter the relative intensities of the excited-state vibrational frequencies of immunocomplexed molecules allowing for unambiguous spectroscopic resolution of all four enantiomeric isomers. This study represents the first example of a high-resolution, fluorescence-based spectroscopic method capable of enantiospecific differentiation.
通过荧光线窄化光谱法研究了高度交叉反应性的抗多环芳烃单克隆抗体与四种非对映体苯并[a]芘四醇（BPTs）的相互作用。结果表明，顺式-BPT和反式-BPT对映体与抗体的相互作用涉及不同的复合物几何结构。这些空间上不同的配体-蛋白质相互作用改变了免疫复合物分子激发态振动频率的相对强度，从而能够对所有四种对映体异构体进行明确的光谱分辨。本研究代表了一种能够进行对映体特异性区分的高分辨率荧光光谱方法的首个实例。
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