Patra Digambara
Department of Chemistry, Indian Institute of Technology-Madras, Chennai 600 036, India.
Luminescence. 2003 Mar-Apr;18(2):97-102. doi: 10.1002/bio.712.
Benzo[a]pyrene causes cancer at cellular level and is widely present in the environment. Conventional spectroscopic methods for analysis of this compound need a pre-separation procedure due to severe spectral overlap from other polycyclic aromatic hydrocarbons. We report a simple method that avoids spectral overlap of benzo[a]pyrene from other impurities or polycyclic aromatic hydrocarbons (PAHs), thus it can easily identify benzo[a]pyrene in a complex PAH mixture. The method could easily identify benzo[a]pyrene in an 18-component PAH mixture. Calibration plots in methanol solution and in micellar media show a good linearity (R > 0.9997) in the benzo[a]pyrene concentration range generally found in the environment. The method gives a detection limit of 1.52 x 10(-9) mol/L in CTAB micellar medium and 2.55 x 10(-9) mol/L in methanol solution. The proposed method is selective, sensitive and fast. The fluorescence response of benzo[a]pyrene is found to be a potential candidate to sense the critical micellar concentration (CMC) of CTAB micelles.
苯并[a]芘在细胞水平上可致癌，且广泛存在于环境中。由于与其他多环芳烃存在严重的光谱重叠，用于分析该化合物的传统光谱方法需要进行预分离程序。我们报道了一种简单的方法，该方法可避免苯并[a]芘与其他杂质或多环芳烃（PAHs）的光谱重叠，因此能够轻松识别复杂PAH混合物中的苯并[a]芘。该方法能够轻松识别18种成分的PAH混合物中的苯并[a]芘。甲醇溶液和胶束介质中的校准曲线在环境中通常发现的苯并[a]芘浓度范围内显示出良好的线性（R>0.9997）。该方法在CTAB胶束介质中的检测限为1.52×10⁻⁹mol/L，在甲醇溶液中的检测限为2.55×10⁻⁹mol/L。所提出的方法具有选择性、灵敏性和快速性。发现苯并[a]芘的荧光响应是检测CTAB胶束临界胶束浓度（CMC）的潜在候选方法。
