Shin Su-Kyoung, Xu Delin, Kwon Hyung-Jin, Suh Joo-Won
Department of Biolog ical Science, Institute of Bioscience and Biotechnology, Myongji University, Yongin, Korea.
FEMS Microbiol Lett. 2006 Jun;259(1):53-9. doi: 10.1111/j.1574-6968.2006.00246.x.
S-adenosylmethionine (SAM), the major methyl donor in diverse biological processes, was previously documented as a regulator for secondary metabolism in Streptomyces. The present study demonstrates that exogenous SAM, in a quantity as low as 10 muM, enhanced streptomycin production and adpA transcription in both Streptomyces griseus wild-type strain and mutant HO1, which displays no ArpA repression on the adpA promoter. SAM also enhanced xylE expression driven by the promoter of adpA or strR in a heterologous host, S. lividans. This report substantiates that exogenous SAM promotes adpA transcription in S. griseus, which is involved in the SAM-mediated promotion of streptomycin, and that the mechanism underlying this event is shared in S. lividans.
S-腺苷甲硫氨酸（SAM）是多种生物过程中的主要甲基供体，此前有文献记载它是链霉菌次生代谢的调节因子。本研究表明，低至10μM的外源SAM可增强灰色链霉菌野生型菌株和突变体HO1中的链霉素产量及adpA转录，突变体HO1对adpA启动子没有ArpA抑制作用。SAM还增强了在异源宿主天蓝色链霉菌中由adpA或strR启动子驱动的xylE表达。本报告证实，外源SAM促进了灰色链霉菌中adpA的转录，这与SAM介导的链霉素促进作用有关，并且这一事件的潜在机制在天蓝色链霉菌中是相同的。
