Wang H, Huang F, Li A, Shao Q, Niu D
Institute of Genetics, Chinese Academy of Sciences, Beijing.
Chin J Biotechnol. 1991;7(3):185-9.
A simple and effective system for the transformation and regeneration of Lycium barbarum L. has been developed. Young stem segments from Lycium barbarum L. were infected by Agrobacterium tumefaciens harboring a vector containing neomycin phosphotransferase II (npt-II) gene derived from non-oncogenic Ti plasmid. Calli originating from young stem segments on selective induction medium could differentiate into buds on selective differentiation medium rapidly and finally developed into whole plants. NPT-II enzyme activity assay and DNA hybridization indicated that the foreign gene had been integrated into the genome of Lycium barbarum L. and could be expressed in plants.
已开发出一种简单有效的宁夏枸杞转化与再生系统。携带源自非致癌Ti质粒的含有新霉素磷酸转移酶II（npt-II）基因载体的根癌农杆菌感染了宁夏枸杞的幼嫩茎段。在选择性诱导培养基上源自幼嫩茎段的愈伤组织在选择性分化培养基上能迅速分化成芽，最终发育成完整植株。NPT-II酶活性测定和DNA杂交表明，外源基因已整合到宁夏枸杞基因组中并能在植株中表达。
