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对橙黄网孢盘菌凝集素耐药的Lewis肺癌细胞亚系中含α(1-3)-半乳糖的天冬酰胺连接聚糖的结构:通过1H-NMR光谱法阐明

Structures of the alpha(1-3)-galactose-containing asparagine-linked glycans of a Lewis lung carcinoma cell subline resistant to Aleuria aurantia agglutinin: elucidation by 1H-NMR spectroscopy.

作者信息

Debray H, Dus D, Wieruszeski J M, Strecker G, Montreuil J

机构信息

Laboratoire de Chimie Biologique, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.

出版信息

Glycoconj J. 1991 Feb;8(1):29-37. doi: 10.1007/BF00731640.

DOI:10.1007/BF00731640
PMID:1668529
Abstract

Four bi-antennary glycan fractions of the N-acetyllactosamine-type, derived from a Lewis lung carcinoma (LL2) cell subline resistant to the Aleuria aurantia agglutinin were studied by 400 MHz 1H-NMR spectroscopy. By this method, their antennae were found to be terminated either by alpha(2-3 or 6)-linked N-acetylneuraminic acid or alpha(1-3)-linked galactose residues. The primary structure of glycans of these four glycopeptide or derived oligosaccharide-alditols has been determined in full detail.

摘要

采用400兆赫的1H-NMR光谱法研究了来自对橙黄网柄菌凝集素具有抗性的Lewis肺癌(LL2)细胞亚系的四种N-乙酰乳糖胺型双触角聚糖组分。通过这种方法,发现它们的触角末端要么是α(2-3或6)-连接的N-乙酰神经氨酸,要么是α(1-3)-连接的半乳糖残基。已详细确定了这四种糖肽或衍生的低聚糖-糖醇聚糖的一级结构。

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Structures of the alpha(1-3)-galactose-containing asparagine-linked glycans of a Lewis lung carcinoma cell subline resistant to Aleuria aurantia agglutinin: elucidation by 1H-NMR spectroscopy.对橙黄网孢盘菌凝集素耐药的Lewis肺癌细胞亚系中含α(1-3)-半乳糖的天冬酰胺连接聚糖的结构:通过1H-NMR光谱法阐明
Glycoconj J. 1991 Feb;8(1):29-37. doi: 10.1007/BF00731640.
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Separation of serum albumin into two fractions: Observations on the nature of the glycoprotein fraction.血清白蛋白分离为两个组分:关于糖蛋白组分性质的观察
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Gas--liquid chromatography and mass spectrometry of methylated and acetylated methyl glycosides. Application to the structural analysis of glycoprotein glycans.甲基化和乙酰化甲基糖苷的气相色谱和质谱分析。在糖蛋白聚糖结构分析中的应用。
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The distribution of repeating [Gal beta 1,4GlcNAc beta 1,3] sequences in asparagine-linked oligosaccharides of the mouse lymphoma cell lines BW5147 and PHAR 2.1.重复的[半乳糖β1,4-N-乙酰葡糖胺β1,3]序列在小鼠淋巴瘤细胞系BW5147和PHAR 2.1的天冬酰胺连接寡糖中的分布
J Biol Chem. 1984 May 25;259(10):6253-60.
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Structures of the O-linked oligosaccharides of the major cell surface sialoglycoprotein of MAT-B1 and MAT-C1 ascites sublines of the 13762 rat mammary adenocarcinoma.13762大鼠乳腺腺癌MAT - B1和MAT - C1腹水亚系主要细胞表面唾液酸糖蛋白的O - 连接寡糖结构
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Isolation and characterization of a family of alpha-D-galactosyl-containing glycopeptides from Ehrlich ascites tumor cells.从艾氏腹水瘤细胞中分离和鉴定含α-D-半乳糖基的糖肽家族。
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