Wang You, Yu Zhi-ming, Song Xiu-xian, Zhang Shan-dong
Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanography, Chinese Academy of Sciences, Qingdao 266071, China.
Huan Jing Ke Xue. 2006 Feb;27(2):274-80.
We studied the effects of fresh tissue and culture medium filtrate of Ulva pertusa (Chlorophyta) and Gracilaria lemaneiformis (Rhodophyta) on growth of 2 causative bloom species: Prorocentrum donghaiense and Alexandum tamarense, in laboratory conditions. Both U. pertusa and G. lemaneiformis, especially their fresh tissues, significantly interfered with the growth of the co-cultured microalgae. P. donghaiense could be completely killed in the bialgal culture, but growth of A. tamarense in it was little affected. Simultaneous assay on the effects of culture medium filtrates showed that the culture filtrate of A. tamarense had algicidal effect on P. donghaiense, while that of P. donghaiense had little effect on growth of A. tamarense. We simulated the interactions between P. donghaiense and A. tamarense in the bialgal culture by using a mathematical model. Results show that the effect of P. donghaiense inhibited by A. tamarense is about 17 times larger than the inhibitory effect of P. donghaiense exerted on A. tamarense. The joint effects of multialgal cultures on microalga were analyzed. Results present that the multialgal cultures of either U. pertusa or G. lemaneiformis on P. donghaiense are synergism, and the joint effect of U. pertusa and P. donghaiense on A. tamarense is additional. However, it was difficult to determine the joint effects of G. lemaneiformis and P. donghaiense on A. tamarense. Results suggest that allelopathy is the most likely reason responsible for the results obtained in this paper.
我们在实验室条件下，研究了孔石莼（绿藻门）和龙须菜（红藻门）的新鲜组织及培养基滤液对两种引发藻华的物种——东海原甲藻和塔玛亚历山大藻生长的影响。孔石莼和龙须菜，尤其是它们的新鲜组织，均显著干扰了共培养微藻的生长。在双藻培养中，东海原甲藻可被完全杀死，但塔玛亚历山大藻的生长几乎未受影响。同时对培养基滤液的作用进行测定表明，塔玛亚历山大藻的培养基滤液对东海原甲藻有杀藻作用，而东海原甲藻的培养基滤液对塔玛亚历山大藻的生长几乎没有影响。我们用数学模型模拟了双藻培养中东海原甲藻和塔玛亚历山大藻之间的相互作用。结果表明，塔玛亚历山大藻对东海原甲藻的抑制作用比东海原甲藻对塔玛亚历山大藻的抑制作用大约大17倍。分析了多藻培养对微藻的联合作用。结果表明，孔石莼或龙须菜与东海原甲藻的多藻培养是协同作用，孔石莼与东海原甲藻对塔玛亚历山大藻的联合作用是相加作用。然而，难以确定龙须菜与东海原甲藻对塔玛亚历山大藻的联合作用。结果表明，化感作用最有可能是导致本文结果的原因。
