Pessi A, Bianchi E, Chiappinelli L, Bonelli F, Tougne C, Lambert P H, Del Giudice G
Peptide Synthesis Unit, SCLAVO SpA, Monterotondo, Rome, Italy.
Parassitologia. 1991 Apr;33(1):79-84.
Multiple Antigen Peptides (MAPs), branched molecules where multiple copies of a desired antigenic sequence are assembled on a small peptide core, have been recently described as an alternative approach to the synthesis of high molecular weight immunogens. In comparison with conventional peptide-carrier conjugates, the MAPs show several advantages, including chemical unambiguity and ease of synthesis. A MAP based on the sequence of the repetitive domain of P. malariae sporozoites was immunogenic in a large number of mouse strains. When covalently linked to the corresponding sequence of the P. falciparum circumsporozoite protein, [NANP]40, the resulting conjugate showed the properties of a multivalent vaccine, overcoming the severe genetic restriction of the [NANP] sequence. A second generation of MAPs including both sequences, with more desirable chemical properties, was equally effective. These compounds represent a promising step towards the development of synthetic, multivalent peptide vaccines against human malaria.
多抗原肽（MAPs）是一种分支分子，其中多个所需抗原序列的拷贝组装在一个小肽核心上，最近被描述为合成高分子量免疫原的一种替代方法。与传统的肽-载体缀合物相比，MAPs具有几个优点，包括化学明确性和易于合成。基于恶性疟原虫子孢子重复结构域序列的MAP在大量小鼠品系中具有免疫原性。当与恶性疟原虫环子孢子蛋白的相应序列[NANP]40共价连接时，所得缀合物显示出多价疫苗的特性，克服了[NANP]序列的严重遗传限制。包括这两个序列且具有更理想化学性质的第二代MAP同样有效。这些化合物代表了朝着开发针对人类疟疾的合成多价肽疫苗迈出的有希望的一步。
