毛细管区带电泳法测定药物中的布洛芬和氟比洛芬
Determination of ibuprofen and flurbiprofen in pharmaceuticals by capillary zone electrophoresis.
作者信息
Hamoudová Rafifa, Pospísilová Marie
机构信息
Charles University, Faculty of Pharmacy, Department of Analytical Chemistry, Heyrovského 1203, CZ-500 05 Hradec Králové, Czech Republic.
出版信息
J Pharm Biomed Anal. 2006 Jun 16;41(4):1463-7. doi: 10.1016/j.jpba.2006.03.024. Epub 2006 May 9.
Capillary zone electrophoresis with spectrophotometric detection was used for the determination of ibuprofen (IB) and flurbiprofen (FL) in pharmaceuticals. The separation was carried out in a fused silica capillary (60 cm x 100 microm i.d. effective length 45 cm) at 30 kV with UV detection at 232 nm. The optimized background electrolyte was 20mM N-(2-acetamido)-2-aminoethanesulfonic acid (ACES) with 20mM imidazole and 10mM alpha-cyclodextrin of pH 7.3. 2-Naphthoxyacetic acid was used as internal standard. A single analysis took less than 5 min. Rectilinear calibration ranges were 2-500 mg l(-1) for IB and 1-60 mg l(-1) for FL. The relative standard deviations (R.S.D.) values (n=6) were 1.53% for IB and 1.29% for FL (for 200 mg l(-1) IB and 10 mg l(-1) FL). This validated method has been successfully applied for the routine analysis of 10 commercially available pharmaceutical preparations (syrup, tablets, cream and gel).
采用毛细管区带电泳-分光光度检测法测定药物中的布洛芬（IB）和氟比洛芬（FL）。在熔融石英毛细管（60 cm×100 μm内径，有效长度45 cm）中，于30 kV电压下进行分离，并在232 nm处进行紫外检测。优化后的背景电解质为20 mM N-（2-乙酰氨基）-2-氨基乙烷磺酸（ACES）、20 mM咪唑和10 mM α-环糊精，pH值为7.3。以2-萘氧基乙酸作为内标。单次分析耗时不到5分钟。IB的线性校准范围为2-500 mg l⁻¹，FL的线性校准范围为1-60 mg l⁻¹。对于200 mg l⁻¹的IB和10 mg l⁻¹的FL，相对标准偏差（R.S.D.）值（n = 6）分别为1.53%和1.29%。该经过验证的方法已成功应用于10种市售药物制剂（糖浆、片剂、乳膏和凝胶）的常规分析。
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