Prado M S, Steppe M, Tavares M F, Kedor-Hackman E R, Santoro M I
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, SP, Brazil.
J Capill Electrophor Microchip Technol. 1999 May-Aug;6(3-4):125-9.
A capillary electrophoresis method for the determination of diclofenac sodium in a commercial and simulated tablet formation has been described. The aim of this research was to develop a rapid, simple, efficient, and economical method to determine diclofenac sodium in pharmaceutials. The analysis was carried out in a bare silica capillary (75 pm i.d.) with a total length of 50 cm (28 cm to the detector) with a buffer solution containing 20 mM sodium tetraborate, pH 9.23. The applied voltage was 20 kV. Detection was achieved by ultraviolet absorption at 276 nm. Acetaminophen was used as an internal standard. The calibration curve was linear to the concentration range from 40.0 to 120.0 microg/mL with a correlation coefficient of 0.9992. The percentage recovery was found to be 103.12 +/- 0.91. The results showed that the method allows the determination of diclofenac sodium in commercially available pharmaceutical preparations.
本文描述了一种用于测定市售片剂和模拟片剂中双氯芬酸钠的毛细管电泳方法。本研究的目的是开发一种快速、简单、高效且经济的方法来测定药物制剂中的双氯芬酸钠。分析在内径为75μm、总长为50cm（至检测器的长度为28cm）的裸硅胶毛细管中进行，缓冲溶液含有20mM硼酸钠，pH 9.23。施加电压为20kV。通过在276nm处的紫外吸收进行检测。对乙酰氨基酚用作内标。校准曲线在40.0至120.0μg/mL的浓度范围内呈线性，相关系数为0.9992。回收率为103.12±0.91。结果表明，该方法可用于测定市售药物制剂中的双氯芬酸钠。
