Iitaka M, Fukasawa N, Yanagisawa M, Hase K, Miura S, Hara Y, Ishii J, Kawazu S, Komeda K
Fourth Department of Internal Medicine, Saitama Medical School, Japan.
J Clin Lab Immunol. 1991 Sep;36(1):33-8.
The effect of cholera toxin (CT) on the thyroid-pituitary axis and the immune system was examined in Bio-Breeding/Tokyo (BB/TKY) rats, which spontaneously develop insulin-dependent diabetes mellitus (DM) and lymphocytic thyroiditis (LT). Intravenous administration of CT (5 micrograms/100 g body weight) every other week starting at 6 weeks of age resulted in a significant decrease in the serum thyrotropin (TSH) level at 12 and 14 weeks of age when compared with saline treated littermates. CT stimulated rat thyroid cells to proliferate in vitro. Furthermore, serum anti-thyroglobulin antibody (ATA) titers were also significantly decreased in 14-week-old rats treated with CT. In vitro ATA production by spleen cells from BB/TKY rats was inhibited by CT. Antibodies to thyroxine were detected in both CT-treated and control rats. It is of interest that the ratio of W3/25+ helper/inducer cells to OX8+ suppressor/cytotoxic cells was significantly decreased in CT-treated rats. However, there was no significant difference in the incidence of DM and LT between the two groups of rats. The present study showed that CT suppressed ATA production both in vivo and in vitro, and had a stimulatory effect on thyrocytes in BB/TKY rats.
在Bio-Breeding/Tokyo（BB/TKY）大鼠中研究了霍乱毒素（CT）对甲状腺-垂体轴和免疫系统的影响，该大鼠可自发发展为胰岛素依赖型糖尿病（DM）和淋巴细胞性甲状腺炎（LT）。从6周龄开始每隔一周静脉注射CT（5微克/100克体重），与生理盐水处理的同窝大鼠相比，12周龄和14周龄时血清促甲状腺激素（TSH）水平显著降低。CT在体外刺激大鼠甲状腺细胞增殖。此外，在14周龄接受CT治疗的大鼠中，血清抗甲状腺球蛋白抗体（ATA）滴度也显著降低。CT抑制了BB/TKY大鼠脾细胞的体外ATA产生。在CT治疗组和对照组大鼠中均检测到抗甲状腺素抗体。有趣的是，CT治疗组大鼠中W3/25+辅助/诱导细胞与OX8+抑制/细胞毒性细胞的比例显著降低。然而，两组大鼠的DM和LT发病率没有显著差异。本研究表明，CT在体内和体外均抑制ATA产生，并对BB/TKY大鼠的甲状腺细胞有刺激作用。
