Foglé-Ansson Margaretha, White Peter, Hermansson Ann, Melhus Asa
Department of Oto-Rhino-Laryngology, KSS, Skövde, Sweden.
APMIS. 2006 Apr;114(4):285-91. doi: 10.1111/j.1600-0463.2006.apm_297.x.
The aim of the present study was to explore whether it was possible to differentiate the clinical course and the otomicroscopic appearance of acute otitis media (AOM) caused by common otitis pathogens in an animal model. Systemic interleukin (IL)-6 levels as early markers for bacterial AOM were also studied. Four groups of rats were inoculated with either Streptococcus pneumoniae, Streptococcus pyogenes, non-typeable Haemophilus influenzae or Moraxella catarrhalis. The animals were monitored by otomicroscopy, photos of the tympanic membrane, cultures and IL-6 detection in serum the following 4 days. The gram-positive S. pneumoniae and S. pyogenes induced severe AOM with opaque effusion behind the tympanic membrane, pronounced dilation of the vessels and spontaneous perforations. The gram-negative H. influenzae and M. catarrhalis induced a less severe infection with cloudy, sometimes foamy effusion, and no spontaneous perforations. With the otomicroscopic findings it was possible to distinguish between infections induced by gram-positive bacteria and gram-negative bacteria. Detection of interleukin-6 in serum appeared to be of limited use for all infections except the pneumococcal AOM, but this needs to be further investigated.
本研究的目的是探讨在动物模型中是否有可能区分由常见中耳炎病原体引起的急性中耳炎（AOM）的临床病程和耳镜检查表现。还研究了全身白细胞介素（IL）-6水平作为细菌性AOM的早期标志物。四组大鼠分别接种肺炎链球菌、化脓性链球菌、不可分型流感嗜血杆菌或卡他莫拉菌。在接下来的4天里，通过耳镜检查、鼓膜照片、培养以及血清中IL-6检测对动物进行监测。革兰氏阳性的肺炎链球菌和化脓性链球菌引起严重的AOM，鼓膜后有不透明积液、血管明显扩张和自发性穿孔。革兰氏阴性的流感嗜血杆菌和卡他莫拉菌引起的感染较轻，积液浑浊，有时呈泡沫状，无自发性穿孔。根据耳镜检查结果，可以区分革兰氏阳性菌和革兰氏阴性菌引起的感染。血清中白细胞介素-6的检测似乎除了肺炎球菌性AOM外，对所有感染的作用有限，但这需要进一步研究。
