Martins Thomas B, Anderson Jeffrey L, Muhlestein Joseph B, Horne Benjamin D, Carlquist John F, Roberts William L, Carlquist John F
Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA.
Am J Clin Pathol. 2006 Jun;125(6):906-13. doi: 10.1309/Q3E6-KF0Q-D3U3-YL6T.
Coronary artery disease (CAD) is the leading cause of death in the United States. Increasing evidence suggests involvement of inflammation in the atherosclerotic process. We examined cytokines and other inflammatory markers in 865 patients with chest pain in whom coronary angiography revealed no evidence of CAD or CAD with or without concomitant myocardial infarction (MI). We developed a multiplexed immunoassay to simultaneously assess the plasma concentrations of 8 cytokines (interferon gamma, interleukin (IL)-2, IL-4, IL-6, IL-8, IL-10, IL-12, and tumor necrosis factor alpha), IL-2r, and soluble CD40 ligand in the patient groups. Concentrations of C-reactive protein (CRP) and IL-18 also were determined. Significant differences (P < .05) between no CAD and combined CAD groups were found for IL-2, IL-4, IL-6, IL-12, and IL-18. When the no CAD group was compared with the group with CAD with subsequent MI, significant differences were found for proinflammatory markers IL-6 (P pound .001), IL-8 (P = .017), and CRP (P pound .001). Cytokine profiles may have a role in differentiating patients with CAD with MI from those with chest pain due to other disorders and in deciphering the role of inflammation in the pathogenesis of CAD.
冠状动脉疾病（CAD）是美国主要的死亡原因。越来越多的证据表明炎症参与了动脉粥样硬化过程。我们检测了865例胸痛患者的细胞因子和其他炎症标志物，这些患者的冠状动脉造影显示无CAD证据或伴有或不伴有心肌梗死（MI）的CAD。我们开发了一种多重免疫测定法，以同时评估患者组中8种细胞因子（干扰素γ、白细胞介素（IL）-2、IL-4、IL-6、IL-8、IL-10、IL-12和肿瘤坏死因子α）、IL-2r和可溶性CD40配体的血浆浓度。还测定了C反应蛋白（CRP）和IL-18的浓度。在无CAD组和合并CAD组之间，IL-2、IL-4、IL-6、IL-12和IL-18存在显著差异（P <.05）。当将无CAD组与随后发生MI的CAD组进行比较时，促炎标志物IL-6（P≤.001）、IL-8（P =.017）和CRP（P≤.001）存在显著差异。细胞因子谱可能在区分患有MI的CAD患者与因其他疾病引起胸痛的患者以及解读炎症在CAD发病机制中的作用方面发挥作用。
