Seki Fumio, Takeda Makoto, Minagawa Hiroko, Yanagi Yusuke
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan.
J Gen Virol. 2006 Jun;87(Pt 6):1643-1648. doi: 10.1099/vir.0.81682-0.
Signalling lymphocyte activation molecule (SLAM) acts as a cellular receptor for Measles virus (MV). The recombinant MV, based on a SLAM-using clinical isolate in which asparagine at position 481 of the haemagglutinin was replaced with tyrosine, was generated. Characterization of this recombinant virus revealed that the N481Y substitution in the haemagglutinin allowed it to utilize CD46 as an alternative receptor, but that its ability to use CD46 was rather low in CD46+ SLAM- cell lines compared with that of the recombinant virus possessing the haemagglutinin of the Edmonston laboratory strain. Thus, an N481Y substitution alone may not be sufficient to make SLAM-using MVs use CD46 efficiently, suggesting that further substitutions in the haemagglutinin are required for them to grow efficiently in CD46+ cells like the Edmonston strain. This may be a reason why few CD46-using MVs are detected in vivo.
信号淋巴细胞激活分子（SLAM）作为麻疹病毒（MV）的细胞受体。基于一株使用SLAM的临床分离株构建了重组MV，该分离株血凝素第481位的天冬酰胺被酪氨酸取代。对这种重组病毒的特性分析表明，血凝素中的N481Y取代使其能够利用CD46作为替代受体，但与具有埃德蒙斯顿实验室株血凝素的重组病毒相比，其在CD46 + SLAM-细胞系中利用CD46的能力相当低。因此，仅N481Y取代可能不足以使使用SLAM的MV有效地利用CD46，这表明血凝素中还需要进一步的取代，以便它们能像埃德蒙斯顿株一样在CD46 +细胞中高效生长。这可能是体内很少检测到使用CD46的MV的原因之一。
