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中华鳖和扬子鳄中具有温度依赖型性别决定的Sox-1、-2、-3同源物的分离与序列分析

Isolation and sequence analysis of the Sox-1, -2, -3 homologs in Trionyx sinensis and Alligator sinensis having temperature-dependent sex determination.

作者信息

Zheng Jifang, Zhu Muyuan

机构信息

Instiute of Geneties, College of Life Sciences, Zhejiang University, Hangzhou 310012, P.R. China.

出版信息

Biochem Genet. 2006 Apr;44(3-4):101-12. doi: 10.1007/s10528-006-9019-0. Epub 2006 May 12.

Abstract

Members of the Sox gene family are characterized by an HMG-box that shows sequence similarity with that of the mouse testis-determining gene Sry. Using degenerate primers PCR, seven and eight HMG-box motifs of Sry-related genes were cloned and sequenced from genomic DNA of Trionyx sinensis (termed TS41-47) and Alligator sinensis (AS41-48) with TSD (temperature-dependent sex determination). Among 15 Sry-related genes, TS41, TS42, AS41, and AS42 shared 80, 72, 81, and 79% amino acid identity, respectively, with each HMG-box domain of the mouse Sox-1, -2, and -3 genes by Blast analysis. Molecular phylogenetic analysis showed that the clustering of TS41-42 and AS41-42 was distant to the clustering of the nonreptilian vertebrate Sox-1, -2, -3 homologs, including fish, amphibian, bird, and mammals. The amino acid identity among TS41-42, AS41-42, and the nonreptilian vertebrate Sox-1, -2, -3 homologs is lower than identities among the Sox-1, -2, -3 homologs, suggesting that the sequence changes in TS41-42 of Trionyx sinensis and AS41-42 of Alligator sinensis might have occurred after the diversification of amniotes.

摘要

Sox基因家族的成员具有一个HMG框,该框与小鼠睾丸决定基因Sry的序列相似。利用简并引物PCR,从中华鳖(称为TS41 - 47)和扬子鳄(AS41 - 48)具有温度依赖型性别决定(TSD)的基因组DNA中克隆并测序了7个和8个与Sry相关基因的HMG框基序。在15个与Sry相关的基因中,通过Blast分析,TS41、TS42、AS41和AS42与小鼠Sox - 1、- 2和- 3基因的每个HMG框结构域分别共享80%、72%、81%和79%的氨基酸同一性。分子系统发育分析表明,TS41 - 42和AS41 - 42的聚类与非爬行类脊椎动物Sox - 1、- 2、- 3同源物(包括鱼类、两栖类、鸟类和哺乳动物)的聚类距离较远。TS41 - 42、AS41 - 42与非爬行类脊椎动物Sox - 1、- 2、- 3同源物之间的氨基酸同一性低于Sox - 1、- 2、- 3同源物之间的同一性,这表明中华鳖的TS41 - 42和扬子鳄的AS41 - 42中的序列变化可能发生在羊膜动物分化之后。

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