The Anhui Provincial Key Laboratory of Biodiversity Conservation and Ecological Security in the Yangtze River Basin, College of Life Science, Anhui Normal University, Wuhu 241000, China.
Int J Mol Sci. 2024 Oct 7;25(19):10771. doi: 10.3390/ijms251910771.
The gene superfamily is widely distributed among higher eukaryotes and plays a significant role in numerous biological processes. However, there is limited knowledge about the structure and function of genes in the critically endangered species , which is endemic to China. A total of 170 genes were identified from the genome, classified into 13 families, and unevenly distributed across 16 chromosomes. Analysis of gene duplication events yielded eight pairs of tandem duplication genes and six pairs of segmental duplication genes. Phylogenetics shows that the genes are evolutionarily conserved. The cis-regulatory elements in the family promoter region reveal their involvement in multiple biological processes. Protein interaction network analysis indicates that the protein interactions of the genes are centered around CLU-3, mainly participating in the regulation of biological processes through the ubiquitination pathway. The expression profile and protein interaction network analysis of genes during sex differentiation and early gonadal development indicate that genes are widely expressed in this process and involves numerous genes and pathways for regulation. This study provides a basis for further investigation of the role of the gene in sex differentiation and gonadal development in .
基因超家族广泛分布于高等真核生物中，在众多生物学过程中发挥着重要作用。然而，对于中国特有的极度濒危物种中的基因结构和功能知之甚少。从基因组中鉴定出了 170 个基因，分为 13 个家族，不均匀分布在 16 条染色体上。基因复制事件分析产生了 8 对串联重复基因和 6 对片段重复基因。系统发育分析表明，这些基因在进化上是保守的。在 家族启动子区域的顺式调控元件揭示了它们参与多个生物学过程。蛋白质相互作用网络分析表明，的基因的蛋白质相互作用以 CLU-3 为中心，主要通过泛素化途径参与生物过程的调节。在性别分化和早期性腺发育过程中 基因的表达谱和蛋白质相互作用网络分析表明，基因在这个过程中广泛表达，并涉及到许多基因和途径的调节。本研究为进一步研究基因在性别分化和性腺发育中的作用提供了基础。
