甲磺酸培氟沙星与牛乳铁蛋白和人血清白蛋白的结合相互作用。
Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin.
作者信息
Fan Ji-cai, Chen Xiang, Wang Yun, Fan Cheng-ping, Shang Zhi-cai
机构信息
Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
出版信息
J Zhejiang Univ Sci B. 2006 Jun;7(6):452-8. doi: 10.1631/jzus.2006.B0452.
Abstract
The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Förster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy.
摘要
采用荧光光谱和吸收光谱研究了甲磺酸培氟沙星（PFLX）在稀水溶液中与牛乳铁蛋白（BLf）和人血清白蛋白（HSA）的结合。通过荧光猝灭法获得结合常数K和结合位点数n。根据Förster型偶极 - 偶极非辐射能量转移机制，还得到了甲磺酸培氟沙星与牛乳铁蛋白以及人血清白蛋白之间的结合距离r和能量转移效率E。利用同步荧光光谱分析了甲磺酸培氟沙星对牛乳铁蛋白和人血清白蛋白构象的影响。
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