Huacuja R L, Delgado N M, Carranco L A, Reyes L R, Rosado G A
Unidad de Inv. Biomédica de Occidente, IMSS, Guadalajara, Jal., México.
Arch Invest Med (Mex). 1990 Oct-Dec;21(4):393-8.
Human spermatozoa and those obtained from mouse epididymis incubated in ethanolic from mouse epididymis incubated in ethanolic extracts of the cortex of Bursera fagaroides became agglutinated and immobilized in 100% with 30% of viability decrease. This double effect occurred in 50% of pig'ssperm and 50% of rabbit sperm when the concentration was increased tenfold. The pattern of progressive motility was affected 80% of rat's spermatocytes without agglutination. The values remained the same when the extracts were partially purified with salts of Zn++ and Ba++ or by filtration in a sephadex g-10 column. Analysis with thin layer chromatography of both purified extracts produced 3 compounds in comparison with the ethanolic unpurified extract. The extract of the plant Bursera fagaroides contains 3 compounds, which apparently are glycosides with a potent activity upon agglutination -immobilization and a low effect upon spermatocytes viability, which might be used as contraceptives .
人类精子以及从小鼠附睾获取的精子，在与裂榄属植物（Bursera fagaroides）皮层乙醇提取物共同孵育后，会发生凝集并失去活性，在100%的样本中活力下降了30%。当浓度增加10倍时，50%的猪精子和50%的兔精子出现了这种双重效应。80%的大鼠精母细胞的渐进性运动模式受到影响，但未发生凝集。当提取物用Zn++和Ba++盐进行部分纯化或通过葡聚糖凝胶G-10柱过滤时，这些数值保持不变。与未纯化的乙醇提取物相比，对两种纯化提取物进行薄层色谱分析产生了3种化合物。裂榄属植物提取物含有3种化合物，它们显然是糖苷，对凝集-失活具有强大活性，对精母细胞活力影响较小，可能用作避孕药。
