Guilbault G G, Rohm T J
Int J Environ Anal Chem. 1975;4(1):51-64. doi: 10.1080/03067317508071101.
Electrodes have been developed for the assay of glucose, urea, amino acids, uric acid, phosphate, nitrate and perchlorate. The electrodes for the organic compounds are enzyme electrodes which are prepared by chemically immobilizing an enzyme over the outside of a conventional ion-selective electrode. These electrodes will be discussed in depth. The progress and the development of the electrodes that show sensitivity and selectivity for phosphate, nitrate and perchlorate will be outlined. The basis of these sensors is a complex of a transition metal of either an analog of thiourea or an organic chelator, such as 1,10-phenanthraline. Such electrodes respond linearly to phosphate, nitrate or perchlorate, and show selectivity over sulphate, halides and acetate. The linear range of all these electrodes is approx. 10(-1)-10(-5) M with a near Nernstian slope and a reproducibility of 1%. The electrodes are stable and can be used continuously.
已开发出用于检测葡萄糖、尿素、氨基酸、尿酸、磷酸盐、硝酸盐和高氯酸盐的电极。用于有机化合物的电极是酶电极，通过将酶化学固定在传统离子选择性电极的外部制备而成。将深入讨论这些电极。将概述对磷酸盐、硝酸盐和高氯酸盐具有灵敏度和选择性的电极的进展和发展情况。这些传感器的基础是过渡金属与硫脲类似物或有机螯合剂（如1,10 - 菲咯啉）的络合物。此类电极对磷酸盐、硝酸盐或高氯酸盐呈线性响应，对硫酸盐、卤化物和乙酸盐具有选择性。所有这些电极的线性范围约为10(-1)-10(-5) M，具有接近能斯特斜率且重现性为1%。这些电极稳定且可连续使用。
