细胞周期蛋白D1基因在头颈部鳞状细胞癌中的扩增与过表达。
Amplification and overexpression of the cyclin D1 gene in head and neck squamous cell carcinoma.
作者信息
Wang X, Pavelic Z P, Li Y Q, Wang L, Gleich L, Radack K, Gluckman J L, Stambrook P J
机构信息
Department of Cell Biology, Neurobiology and Anatomy, College of Medicine, University of Cincinnati, 231 Bethesda Avenue, Cincinnati, Ohio 45267-0521, USA.
出版信息
Clin Mol Pathol. 1995 Oct;48(5):M256-9. doi: 10.1136/mp.48.5.m256.
Aims-To determine cyclin D1 gene amplification and expression levels in head and neck squamous cell carcinoma (HNSCC) patients.Methods-Total RNA and genomic DNA were isolated from 40 samples of HNSCC tissue and matched normal tissue and were hybridised with a cyclin D1 cDNA probe. Northern and Southern analyses were used to detect mRNA overexpression and cyclin D1 gene amplification, respectively.Results-15 of the 40 HNSCC samples examined (38%) showed cyclin D1 gene amplification. Of these 15 samples, all 13 from which RNA was available showed increased cyclin D1 expression.Conclusions-HNSCC patients with both amplification and overexpression of the cyclin D1 gene are at greater risk than patients who showed no cyclin D1 gene amplification; amplification and over-expression of the cyclin D1 gene may play an important role in the progression of HNSCC and in clinical outcome.
目的——确定头颈部鳞状细胞癌（HNSCC）患者中细胞周期蛋白D1基因的扩增情况及表达水平。方法——从40份HNSCC组织样本及配对的正常组织中提取总RNA和基因组DNA，并与细胞周期蛋白D1 cDNA探针杂交。分别采用Northern和Southern分析检测mRNA过表达及细胞周期蛋白D1基因扩增情况。结果——在所检测的40份HNSCC样本中，有15份（38%）显示细胞周期蛋白D1基因扩增。在这15份样本中，所有可获取RNA的13份样本均显示细胞周期蛋白D1表达增加。结论——细胞周期蛋白D1基因同时存在扩增和过表达的HNSCC患者比未出现细胞周期蛋白D1基因扩增的患者风险更高；细胞周期蛋白D1基因的扩增和过表达可能在HNSCC的进展及临床结局中起重要作用。
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