Hinds P W, Dowdy S F, Eaton E N, Arnold A, Weinberg R A
Whitehead Institute for Biomedical Research, 9 Cambridge Center, MA 02142.
Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):709-13. doi: 10.1073/pnas.91.2.709.
The cyclin D1 (PRAD1, CCND1) gene is affected by translocations and amplification in the genomes of a number of human tumors, suggesting that these changes confer growth advantage on developing tumor cell clones. We show here that in cultured cells, a cDNA clone of the cyclin D1 gene can contribute to cell transformation by complementing a defective adenovirus E1A oncogene. In such cells, this candidate oncogene indeed functions like an oncogene, suggesting a similar role in tumor progression in vivo.
细胞周期蛋白D1（PRAD1，CCND1）基因在许多人类肿瘤基因组中受到易位和扩增的影响，这表明这些变化赋予了正在发育的肿瘤细胞克隆生长优势。我们在此表明，在培养细胞中，细胞周期蛋白D1基因的一个cDNA克隆可通过补充缺陷型腺病毒E1A癌基因来促进细胞转化。在这类细胞中，这个候选癌基因确实发挥着癌基因的作用，提示其在体内肿瘤进展中可能具有类似作用。
