Monges G, Biagini P, Cantaloube J F, De Micco P, Parriaux D, Seitz J F, Delpero J R, Hassoun J
Laboratory of Anatomical Pathology, Paoli Calmettes Institute, 232 Boulevard de Sainte Marguerite, 13009 Marseille, France.
Clin Mol Pathol. 1996 Jun;49(3):M159-65. doi: 10.1136/mp.49.3.m159.
Aims-(1) To investigate the expression of the four main hormones of the digestive tract by performing reverse transcription polymerase chain reaction (RT-PCR) on a series of samples, comprising tumoral and healthy colonic tissues, hepatic metastases and colonic cell line samples; and (2) to study the patterns of labelling obtained with serological and morphological markers.Methods-After extraction and reverse transcription, gastrin, somatostatin, cholecystokinin (CCK) and transforming growth factor alpha (TGFalpha) mRNAs were detected by PCR and nested PCR using specific primers. The corresponding proteins were detected by immunohistochemistry.Results-The cell lines expressed all four mRNAs. Gastrin mRNA was present in most tumoral and metastatic samples, while the somatostatin transcript was detected in all samples and was frequently overexpressed in the normal colon. TGFalpha mRNA was expressed systematically in tumours of the right and transverse colon, but not in those located in the left colon; the expression of CCK mRNA was systematically absent in the left colon.Conclusions-The data presented here shed some light on the transcriptional events involved in the production of the various hormones present in the gastrointestinal tract, in both healthy and tumoral tissues. The various mRNAs expressed in cell lines are therefore not systematically expressed in the human pathology.
目的——（1）通过对一系列样本进行逆转录聚合酶链反应（RT-PCR），包括肿瘤性和健康结肠组织、肝转移灶及结肠细胞系样本，来研究消化道四种主要激素的表达；（2）研究血清学和形态学标志物的标记模式。方法——提取和逆转录后，使用特异性引物通过PCR和巢式PCR检测胃泌素、生长抑素、胆囊收缩素（CCK）和转化生长因子α（TGFα）的mRNA。通过免疫组织化学检测相应蛋白。结果——细胞系表达所有四种mRNA。胃泌素mRNA存在于大多数肿瘤性和转移性样本中，而生长抑素转录本在所有样本中均被检测到，且在正常结肠中常过度表达。TGFα mRNA在右半结肠和横结肠肿瘤中均有系统性表达，但在左半结肠肿瘤中无表达；CCK mRNA在左半结肠中系统性缺失。结论——本文所呈现的数据揭示了健康和肿瘤组织中胃肠道存在的各种激素产生过程中涉及的转录事件。因此，细胞系中表达的各种mRNA在人类病理学中并非都有系统性表达。
