Wang Lei, Ji Guang, Zheng Pei-Yong, Long Ai-Hua
Laboratory of Fatty Liver Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Zhong Xi Yi Jie He Xue Bao. 2006 May;4(3):281-4. doi: 10.3736/jcim20060312.
To establish a model of alcoholic liver fibrosis (ALF) in rats induced by complex factors.
Forty-seven healthy male rats were divided into three groups: normal control group (n=12), minor CCl4 group (n=12) and complex factors group (n=27). The rats in the complex factors group were fed a complex diet including alcohol, corn oil and pyrazole, and administered with intraperitoneal injection of minor CCl4 to induce ALF. During induction process, the histopathological changes of liver tissue and the values of liver-to-body weight ratio were both observed regularly. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyltransferase (gamma-GT) in these three groups were all examined at the 12th week of the induction process.
At the 12th week of the induction process, the model of ALF induced by complex factors was successfully established in rats, and the histopathological presentations showed alcoholic fatty liver, hepatitis and liver fibrosis in a sequence along with the induction process. The value of liver-to-body weight ratio and the serum levels of ALT, AST and gamma-GT of rats in the complex factors group were all significantly different from those in the other two groups.
It is a steady and effective way to induce ALF in rats with complex diet and minor CCI4 injection.
建立复合因素诱导大鼠酒精性肝纤维化（ALF）模型。
将47只健康雄性大鼠分为三组：正常对照组（n = 12）、小剂量四氯化碳组（n = 12）和复合因素组（n = 27）。复合因素组大鼠给予含酒精、玉米油和吡唑的复合饲料，并腹腔注射小剂量四氯化碳诱导ALF。诱导过程中，定期观察肝组织的组织病理学变化及肝体比数值。在诱导过程的第12周检测这三组大鼠血清中的丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和γ-谷氨酰转移酶（γ-GT）水平。
诱导过程第12周，成功建立了复合因素诱导大鼠ALF模型，组织病理学表现随诱导过程依次呈现酒精性脂肪肝、肝炎和肝纤维化。复合因素组大鼠的肝体比数值及血清ALT、AST和γ-GT水平与其他两组相比均有显著差异。
采用复合饲料及小剂量注射四氯化碳诱导大鼠ALF是一种稳定有效的方法。
