Prabhu S, Jainu Mallika, Sabitha K E, Shyamala Devi C S
Department of Biochemistry, University of Madras, A.C. Tech., Guindy campus, Chennai-600 025, India.
Vascul Pharmacol. 2006 Jun;44(6):519-25. doi: 10.1016/j.vph.2006.03.012. Epub 2006 May 11.
Mangiferin, from the leaves of Mangifera indica Linn., has been suggested as useful in the treatment of cardiovascular disorders. In the present study this drug was examined on the alteration of cardiac energy metabolism in isoproterenol (ISPH) administered myocardial infarcted rats. ISPH (20 mg/kg b.w.), which was administered s.c. twice at an interval of 24 h, caused a significant decrease in the activities of TCA cycle enzymes and antioxidant defense enzymes with a concomitant increase in the lipid peroxidation of heart mitochondria in rat model. The ATP production and the oxidation of succinate in State 3 and 4 decreased significantly in the cardiac mitochondria of ISPH administered rats. These functional alterations were supported by severe modifications in mitochondrial ultrastructure. Pretreatment with mangiferin (100 mg/kg b.w. i.p.) for 28 days prevented these mitochondrial alterations, oxidation with energy metabolism and restored the TCA cycle enzyme activities to near normal values following ISPH administration. The structural integrity of the heart was protected by mangiferin in ISPH administered rats when compared to the untreated controls. The present findings suggest that the protective effect of mangiferin can be attributed to its reducing effect on oxidative damage and activation of mitochondrial energy metabolism. These results could be useful to study and understand the cellular events involved in this cardioprotective mechanism of mangiferin. Our studies of mangiferin on heart failure may have important implication for future therapeutic approaches involving in the prevention of cardiovascular diseases.
芒果苷，从芒果叶中提取，已被认为对治疗心血管疾病有用。在本研究中，该药物用于研究其对异丙肾上腺素（ISPH）诱导的心肌梗死大鼠心脏能量代谢变化的影响。ISPH（20mg/kg体重），皮下注射，间隔24小时注射两次，导致大鼠模型心脏线粒体中三羧酸循环酶和抗氧化防御酶的活性显著降低，同时脂质过氧化增加。在ISPH处理的大鼠心脏线粒体中，状态3和状态4下的ATP生成以及琥珀酸氧化显著降低。这些功能改变得到了线粒体超微结构严重改变的支持。用芒果苷（100mg/kg体重，腹腔注射）预处理28天可防止这些线粒体改变、能量代谢氧化，并在ISPH给药后将三羧酸循环酶活性恢复到接近正常水平。与未处理的对照组相比，芒果苷可保护ISPH处理大鼠的心脏结构完整性。目前的研究结果表明，芒果苷的保护作用可归因于其对氧化损伤的减轻作用和线粒体能量代谢的激活。这些结果可能有助于研究和理解芒果苷这种心脏保护机制中涉及的细胞事件。我们对芒果苷在心力衰竭方面的研究可能对未来预防心血管疾病的治疗方法具有重要意义。
