Yang Lijun, Tada Yoshifumi, Yamamoto Masayuki P, Zhao Hui, Yoshikawa Masaaki, Takaiwa Fumio
Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki 305-8602, Japan.
FEBS Lett. 2006 May 29;580(13):3315-20. doi: 10.1016/j.febslet.2006.04.092. Epub 2006 May 8.
RPLKPW is a potent anti-hypertensive peptide designed according to the structure of ovokinin(2-7) (RADHPF). In this study, we generated transgenic rice plants that accumulate the RPLKPW peptide as a fusion protein with the rice storage protein glutelin. The engineered peptide is expressed under the control of endosperm-specific glutelin promoters and specifically accumulates in seeds. Oral administration of either the RPLKPW-glutelin fraction or transgenic rice seeds to spontaneously hypertensive rats (SHRs) significantly reduced systolic blood pressures. These results suggest the possible application of transgenic rice seed as a nutraceutical delivery system and specifically for administration of active peptides in hypertension.
RPLKPW是一种根据卵激肽(2 - 7)(RADHPF)的结构设计的强效抗高血压肽。在本研究中，我们培育了转基因水稻植株，该植株积累与水稻贮藏蛋白谷蛋白融合的RPLKPW肽。工程化肽在胚乳特异性谷蛋白启动子的控制下表达，并特异性地积累在种子中。给自发性高血压大鼠(SHRs)口服RPLKPW -谷蛋白组分或转基因水稻种子均可显著降低收缩压。这些结果表明转基因水稻种子作为一种营养保健品递送系统的潜在应用，特别是用于高血压活性肽的给药。
