Pant Ganesh Raj, Lunt Ross A, Rootes Chris L, Daniels Peter W
Central Veterinary Laboratory, Kathmandu, Nepal.
Comp Immunol Microbiol Infect Dis. 2006 Mar-May;29(2-3):166-75. doi: 10.1016/j.cimid.2006.03.003.
A regional survey was conducted in Nepal for antibody to Japanese encephalitis virus (JEV) in domestic animals. Sera from pigs, and limited numbers of ducks and horses were collected from 16 districts in 2002-2003 and subjected to three serological tests. Of 270 porcine sera tested by C-ELISA, 55% were found positive for the presence of antibodies against Japanese encephalitis virus. Additional testing for IgM antibody to JEV revealed less than 2% of C-ELISA positive sera had evidence of recent JEV infection. Plaque reduction neutralisation tests (PRNT) using JEV, Murray Valley encephalitis (MVEV) and Kunjin (KUNV) viruses implicated JEV as the flavivirus associated with the observed antibody response in most sero-positive pigs. However, eight porcine sera with predominant neutralising antibody for KUNV (an Australasian subtype of West Nile Virus) provided evidence for the circulation of West Nile virus in Nepal.
在尼泊尔针对家畜开展了一项日本脑炎病毒（JEV）抗体的区域性调查。2002年至2003年期间，从16个地区采集了猪、数量有限的鸭和马的血清，并进行了三项血清学检测。在通过C-ELISA检测的270份猪血清中，55%被发现针对日本脑炎病毒抗体呈阳性。对JEV IgM抗体的进一步检测显示，C-ELISA阳性血清中不到2%有近期JEV感染的证据。使用JEV、墨累谷脑炎病毒（MVEV）和库京病毒（KUNV）进行的蚀斑减少中和试验（PRNT）表明，JEV是与大多数血清阳性猪中观察到的抗体反应相关的黄病毒。然而，八份对KUNV（西尼罗河病毒的一种澳大拉西亚亚型）具有主要中和抗体的猪血清，为西尼罗河病毒在尼泊尔的传播提供了证据。
