寄生虫糖缀合物。第16部分：来自利什曼原虫脂磷壁酸聚糖的二糖以及磷酸化二糖和三糖的合成。

Parasite glycoconjugates. Part 16: Synthesis of a disaccharide and phosphorylated di- and tri-saccharides from Leishmania lipophosphoglycan.

作者信息

Ross Andrew J, Sizova Olga V, Nikolaev Andrei V

机构信息

Faculty of Life Sciences, Division of Biological Chemistry and Molecular Microbiology, University of Dundee, UK.

出版信息

Carbohydr Res. 2006 Aug 14;341(11):1954-64. doi: 10.1016/j.carres.2006.03.026. Epub 2006 May 15.

DOI:10.1016/j.carres.2006.03.026

PMID:16697981

Abstract

A neutral disaccharide beta-D-Galp-(1-->4)-alpha-D-Manp and phosphorylated di- and tri-saccharides beta-D-Galp-(1-->3)-[H(2)PO(3)-6]-beta-D-Galp-OCH(2)CHCH(2) and beta-D-Galp-(1-->3)-[H(2)PO(3)-6]-beta-D-Galp-(1-->4)-alpha-D-Manp, which are fragments of the phosphoglycan portion of the surface lipophosphoglycan from Leishmania donovani (the disaccharide) or Leishmania major (all three compounds), were prepared and used as TLC standards to help the identification and differentiation of the elongating and branching beta-D-galactosyl transferase activities in Leishmania. The phosphosaccharides were synthesised using the H-phosphonate method for phosphorylation.

摘要

一种中性二糖β-D-半乳糖基-(1→4)-α-D-甘露糖基以及磷酸化的二糖和三糖β-D-半乳糖基-(1→3)-[H₂PO₃-6]-β-D-半乳糖基-O[CH₂]₈CHCH₂和β-D-半乳糖基-(1→3)-[H₂PO₃-6]-β-D-半乳糖基-(1→4)-α-D-甘露糖基，它们分别是杜氏利什曼原虫（二糖）或硕大利什曼原虫（所有三种化合物）表面脂磷壁酸聚糖磷糖部分的片段，已被制备并用作薄层色谱标准品，以帮助鉴定和区分利什曼原虫中延伸和分支的β-D-半乳糖基转移酶活性。这些磷酸糖是使用H-膦酸酯法进行磷酸化合成的。