Kallivretaki Evangelia, Eggen Rik, Neuhauss Stephan, Alberti Martin, Kausch Ulf, Segner Helmut
Centre for Fish and Wildlife Health, University of Bern, Länggass-strasse 122, Post Box 8466, CH-3001 Bern, Switzerland.
Mar Environ Res. 2006 Jul;62 Suppl:S187-90. doi: 10.1016/j.marenvres.2006.04.003. Epub 2006 Apr 7.
In zebrafish, two isoforms of the aromatase gene exist, namely cyp19a1 and cyp19a2, expressed predominantly in the gonads and brain, respectively. In this study, we focus on characterizing the specificity of antibodies against the aromatase isoforms, and on (xeno)estrogen-induced changes of individual cyp19a2 mRNA concentrations in the brains of adult male zebrafish. Among three polyclonal antibodies studied, the one against CYP19A2 was found to be specific in Western blots and immunohistochemistry. Real-time RT-PCR analyses revealed strong interindividual variation of cyp19a2 levels in the brains of adult male zebrafish. After a three-week-exposure to (xeno)estrogens, mean values of cyp19a2 mRNA levels tended to increase, with significant induction at 200 ng 17beta-estradiol/L, but interindividual variation of cyp19a2 expression was maintained.
在斑马鱼中，存在两种芳香化酶基因的同工型，即cyp19a1和cyp19a2，分别主要在性腺和大脑中表达。在本研究中，我们着重于表征针对芳香化酶同工型抗体的特异性，以及（异源）雌激素诱导成年雄性斑马鱼大脑中单个cyp19a2 mRNA浓度的变化。在所研究的三种多克隆抗体中，发现针对CYP19A2的抗体在蛋白质免疫印迹和免疫组织化学中具有特异性。实时逆转录聚合酶链反应分析显示成年雄性斑马鱼大脑中cyp19a2水平存在强烈的个体间差异。在暴露于（异源）雌激素三周后，cyp19a2 mRNA水平的平均值趋于增加，在200 ng 17β-雌二醇/升时有显著诱导，但cyp19a2表达的个体间差异依然存在。
