Mizutani Kazuo, Terasaki Paul I, Shih Remi N J, Pei Rui, Ozawa Miyuki, Lee Jarhow
Terasaki Foundation Laboratory, Los Angeles, CA 90064, USA.
Hum Immunol. 2006 Mar;67(3):223-9. doi: 10.1016/j.humimm.2006.02.007. Epub 2006 Mar 31.
Antibodies to MICA and MICB antigens were sought in the sera of 139 kidney transplant recipients. MICA*001, *002, *007, 008, and MICB002 antigens were produced in Escherichia coli and then tested using enzyme-linked immunosorbent assay plates. Among 35 normal sera, 6% had MIC antibodies, and among 14 sera from pregnant women, 21% had MIC antibodies. Among 34 patients with functioning transplants with human leukocyte antigen (HLA) antibodies, 24% had MIC antibodies, and 19% of 32 patients without HLA antibodies had MIC antibodies. Among 46 patients who lost grafts with HLA antibodies, 26% had MIC antibodies, and among 27 failed patients without HLA antibodies, 37% had MIC antibodies. We conclude that antibodies to MIC are produced in the course of immunization by pregnancies and kidney transplants. They also occurred more frequently in rejected patients (30%) than in those with functioning grafts (21%).
在139名肾移植受者的血清中寻找针对MICA和MICB抗原的抗体。MICA*001、*002、*007、008和MICB002抗原在大肠杆菌中产生，然后使用酶联免疫吸附测定板进行检测。在35份正常血清中，6%含有MIC抗体；在14份孕妇血清中，21%含有MIC抗体。在34名具有人类白细胞抗原（HLA）抗体的移植功能正常的患者中，24%含有MIC抗体；在32名无HLA抗体的患者中，19%含有MIC抗体。在46名因HLA抗体而移植失败的患者中，26%含有MIC抗体；在27名无HLA抗体的移植失败患者中，37%含有MIC抗体。我们得出结论，MIC抗体是在妊娠和肾移植免疫过程中产生的。它们在移植排斥患者（30%）中出现的频率也高于移植功能正常的患者（21%）。
