Ge Chunmin, Cui Xia, Wang Yonghong, Hu Yuxin, Fu Zhiming, Zhang Dongfen, Cheng Zhukuan, Li Jiayang
State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Cell Res. 2006 May;16(5):446-56. doi: 10.1038/sj.cr.7310056.
Polyamines are implicated in regulating various developmental processes in plants, but their exact roles and how they govern these processes still remain elusive. We report here an Arabidopsis bushy and dwarf mutant, bud2, which results from the complete deletion of one member of the small gene family that encodes S-adenosylmethionine decarboxylases (SAMDCs) necessary for the formation of the indispensable intermediate in the polyamine biosynthetic pathway. The bud2 plant has enlarged vascular systems in inflorescences, roots, and petioles, and an altered homeostasis of polyamines. The double mutant of bud2 and samdc1, a knockdown mutant of another SAMDC member, is embryo lethal, demonstrating that SAMDCs are essential for plant embryogenesis. Our results suggest that polyamines are required for the normal growth and development of higher plants.
多胺参与调控植物的各种发育过程，但其确切作用以及它们如何控制这些过程仍不清楚。我们在此报道一种拟南芥丛生矮化突变体bud2，它是由一个小基因家族的一个成员完全缺失导致的，该家族编码多胺生物合成途径中不可或缺的中间体形成所必需的S-腺苷甲硫氨酸脱羧酶（SAMDCs）。bud2植株在花序、根和叶柄中具有扩大的维管系统，并且多胺的稳态发生改变。bud2与另一个SAMDC成员的敲除突变体samdc1的双突变体胚胎致死，表明SAMDCs对植物胚胎发生至关重要。我们的结果表明，多胺是高等植物正常生长和发育所必需的。
