Chen Jiang Hua, Pang Ji Liang, Wang Li Lin, Luo Yong Hai, Li Xin, Cao Xiang Ling, Lin Kui, Ma Wei, Hu Xiao He, Luo Da
National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, and Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Cell Res. 2006 May;16(5):499-506. doi: 10.1038/sj.cr.7310061.
Although much progress has been made in understanding how floral organ identity is determined during the floral development, less is known about how floral organ is elaborated in the late floral developmental stages. Here we describe a novel floral mutant, wrinkled petals and stamens1 (wps1), which shows defects in the development of petals and stamens. Genetic analysis indicates that wps1 mutant is corresponding to a single recessive locus at the long arm of chromosome 3. The early development of floral organs in wps1 mutant is similar to that in wild type, and the malfunction of the mutant commences in late developmental stages, displaying a defect on the appearance of petals and stamens. In the mature flower, petals and stamen filaments in the mutant are wrinkled or folded, and the cellular morphology under L1 layer of petals and stamen filaments is abnormal. It is found that the expression patterns of floral organ identity genes are not affected in wps1 mutants compared with that of wild type, consistent with the unaltered development of all floral organs. Furthermore, the identities of epidermal cells in different type of petals are maintained. The histological analysis shows that in wps1 flowers all petals are irregularly folded, and there are knotted structures in the petals, while the shape and arrangement of inner cells are malformed and unorganized. Based on these results, we propose that Wps1 acts downstream to the class B floral organ identity genes, and functions to modulate the cellular differentiation during the late flower developmental stages.
尽管在理解花发育过程中花器官身份如何确定方面已经取得了很大进展，但对于花器官在花发育后期如何细化却知之甚少。在这里，我们描述了一种新的花突变体，皱缩花瓣和雄蕊1（wps1），它在花瓣和雄蕊的发育中表现出缺陷。遗传分析表明，wps1突变体对应于3号染色体长臂上的一个单隐性位点。wps1突变体花器官的早期发育与野生型相似，突变体的功能障碍始于发育后期，在花瓣和雄蕊的外观上表现出缺陷。在成熟花中，突变体的花瓣和雄蕊花丝皱缩或折叠，花瓣和雄蕊花丝L1层下的细胞形态异常。研究发现，与野生型相比，花器官身份基因在wps1突变体中的表达模式不受影响，这与所有花器官的发育未改变一致。此外，不同类型花瓣中表皮细胞的身份得以维持。组织学分析表明，在wps1花中，所有花瓣都不规则折叠，花瓣中有打结结构，而内部细胞的形状和排列畸形且无序。基于这些结果，我们提出Wps1在B类花器官身份基因的下游起作用，并在花发育后期调节细胞分化。
