Guo Lan, Yin Benliang, Zhou Junli, Li Xueyong, Deng Xing Wang
National Institute of Biological Science, Zhongguancun Life Science Park, Beijing 102206, China.
Cell Res. 2006 May;16(5):519-27. doi: 10.1038/sj.cr.7310063.
In addition to DNA sequence information, site-specific histone modifications are another important determinant of gene expression in a eukaryotic organism. We selected four modification sites in common histones that are known to significantly impact chromatin function and generated monoclonal or polyclonal antibodies that recognize each of those site-specific modifications. We used these antibodies to demonstrate that the site-specific histone modification levels remain relatively constant in different organs of the same organism. We also compared the levels of selected histone modifications among several representative organisms and found that site-specific modifications are highly variable among different organisms, providing new insight into the evolutionary divergence of specific histone modifications.
除了DNA序列信息外，位点特异性组蛋白修饰是真核生物中基因表达的另一个重要决定因素。我们在常见组蛋白中选择了四个已知会显著影响染色质功能的修饰位点，并生成了识别每个位点特异性修饰的单克隆或多克隆抗体。我们使用这些抗体证明，在同一生物体的不同器官中，位点特异性组蛋白修饰水平保持相对恒定。我们还比较了几种代表性生物体中选定组蛋白修饰的水平，发现位点特异性修饰在不同生物体之间高度可变，这为特定组蛋白修饰的进化差异提供了新的见解。
