Yli-Pirilä Terhi, Kusnetsov Jaana, Hirvonen Maija-Riitta, Seuri Markku, Nevalainen Aino
National Public Health Institute, Department of Environmental Health, Kuopio, Finland.
Can J Microbiol. 2006 Apr;52(4):383-90. doi: 10.1139/w05-131.
Dampness, moisture, and mold in buildings are associated with adverse health outcomes. In addition to fungi and bacteria, amoebae have been found in moisture-damaged building materials. Amoebae and a growing list of bacteria have been shown to have mutual effects on each other's growth, but the interactions between amoebae and microbes common in moisture-damaged buildings have not been reported. We co-cultivated the amoeba Acanthamoeba polyphaga with bacteria and fungi isolated from moisture-damaged buildings in laboratory conditions for up to 28 days. The microbes selected were the bacteria Streptomyces californicus, Bacillus cereus, and Pseudomonas fluorescens, and the fungi Stachybotrys chartarum, Aspergillus versicolor, and Penicillium spinulosum. Fungi and bacteria generally benefited from the presence of the amoebae, whereas the growth of amoebae was hindered by Streptomyces californicus, Stachybotrys chartarum, and Bacillus cereus. Pseudomonas fluorescens slightly enhanced amoebae viability. Amoebae were indifferent to the presence of Aspergillus versicolor and Penicillium spinulosum. Thus, our results show that amoebae can alter the survival and growth of some microbes in moisture-damaged buildings.
建筑物中的潮湿、湿气和霉菌与不良健康后果相关。除了真菌和细菌外，在受潮损坏的建筑材料中还发现了变形虫。变形虫与越来越多的细菌已被证明对彼此的生长有相互影响，但变形虫与受潮损坏建筑物中常见微生物之间的相互作用尚未见报道。我们在实验室条件下将多食棘阿米巴变形虫与从受潮损坏建筑物中分离出的细菌和真菌共同培养长达28天。所选的微生物是加利福尼亚链霉菌、蜡样芽孢杆菌和荧光假单胞菌，以及枝孢霉、杂色曲霉和微小青霉。真菌和细菌通常受益于变形虫的存在，而加利福尼亚链霉菌、枝孢霉和蜡样芽孢杆菌则阻碍了变形虫的生长。荧光假单胞菌略微提高了变形虫的活力。变形虫对杂色曲霉和微小青霉的存在无动于衷。因此，我们的结果表明，变形虫可以改变受潮损坏建筑物中某些微生物的存活和生长。
