Carnieli Junior Pedro, Ventura Armando Moraes, Durigon Edison Luiz
Institute Pasteur São Paulo, São Paulo, SP, Brazil.
Rev Soc Bras Med Trop. 2006 Mar-Apr;39(2):159-62. doi: 10.1590/s0037-86822006000200005. Epub 2006 May 5.
A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.
用巴斯德病毒株制备了地高辛标记的探针，用于检测狂犬病病毒N基因。探针杂交是从通过逆转录聚合酶链反应获得的扩增N基因进行的，逆转录聚合酶链反应（RT-PCR）和杂交的结果显示完全一致。在RT-PCR扩增产物中进行杂交，进一步提高了该技术的灵敏度，并赋予诊断特异性。一旦成本与传统狂犬病诊断技术相当，本文所述技术将对狂犬病诊断实验室有用。
