Yu W-L, Cheng K-C, Chi C-J, Chen H-E, Chuang Y-C, Wu L-T
Department of Critical Care Medicine, Chi-Mei Medical Center, Yungkang, Taiwan.
Clin Microbiol Infect. 2006 Jun;12(6):579-82. doi: 10.1111/j.1469-0691.2006.01384.x.
Enterobacter cloacae (n = 110) isolates from a district hospital in Taiwan were screened for extended-spectrum beta-lactamases (ESBLs). In total, 17 ESBL-producers were identified, based on the combination-disk synergy test using cefotaxime and ceftazidime +/- clavulanic acid. Investigation of ESBL genes in 33 ceftazidime-resistant isolates revealed the SHV-12 gene in the same 17 ESBL-producers. In addition, one isolate also carried the CTX-M-3 gene, and two isolates also carried the CTX-M-9 gene. No major epidemic clone of ESBL-producers was identified by pulsed-field gel electrophoresis. Routine screening for the ESBL phenotype, focusing on ceftazidime-resistant E. cloacae, should be undertaken in this area.
对台湾一家地区医院分离出的110株阴沟肠杆菌进行超广谱β-内酰胺酶（ESBLs）筛查。基于使用头孢噻肟和头孢他啶±克拉维酸的组合药敏试验，共鉴定出17株产ESBLs菌株。对33株头孢他啶耐药菌株的ESBL基因进行研究，发现同样的17株产ESBLs菌株中存在SHV-12基因。此外，一株菌株还携带CTX-M-3基因，两株菌株还携带CTX-M-9基因。通过脉冲场凝胶电泳未鉴定出产ESBLs菌株的主要流行克隆。该地区应针对头孢他啶耐药的阴沟肠杆菌进行ESBL表型的常规筛查。
