离子色谱法测定鳙鱼（鳙）肌原纤维三磷酸腺苷酶活性
Determination of the big head carp myofibrillar (Aristichthys nobilis) adenosine triphosphatase activity by ion chromatography.
作者信息
Gao Rui-chang, Xue Chang-hu, Yuan Li, Li Zhao-jie, Xue Yong, Cui Fengxia, Sun Yan
机构信息
College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China.
出版信息
J Chromatogr A. 2006 Jun 23;1118(2):278-80. doi: 10.1016/j.chroma.2006.04.066. Epub 2006 May 15.
A method for the determination of adenosine triphosphatase (ATPase) activity of myofibrils of big head carp by using ion chromatography was introduced. Adenosine triphosphate (ATP), adenosine diphosphate (ADP) and orthophosphate (Pi) were separated completely. Recoveries for ATP, ADP and Pi were 98+/-5%, 97+/-4% and 98+/-5%, respectively. Pi liberated from ATP during reaction was monitored by ion chromatography using the suggested method. This method was applicable to the determination of myofibrils ATPase activity for quick quality evaluation of surimi.
介绍了一种利用离子色谱法测定鳙鱼肌原纤维三磷酸腺苷酶（ATPase）活性的方法。三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和正磷酸盐（Pi）被完全分离。ATP、ADP和Pi的回收率分别为98±5%、97±4%和98±5%。反应过程中从ATP释放的Pi采用建议的方法通过离子色谱法进行监测。该方法适用于肌原纤维ATPase活性的测定，以快速评估鱼糜的质量。
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