Bhattarai Shanta Raj, Yi Ho-Keun, Bhattarai Narayan, Hwang Pyoung Han, Kim Hak Yong
Department of Bionanosystem Engineering, Chonbuk National University, Chonju, South Korea.
Acta Biomater. 2006 Mar;2(2):207-12. doi: 10.1016/j.actbio.2005.10.007. Epub 2006 Jan 6.
The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co-L-lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the beta-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by beta-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy.
在单层培养中，细胞通过阳离子脂质介导摄取质粒DNA的过程，被嵌段共聚物聚（对二氧六环酮 - 共 - L - 丙交酯）-b-聚（乙二醇）（PPDO/PLLA-b-PEG）的水溶液显著增强。使用编码β-半乳糖苷酶基因的DNA进行质粒摄取研究，并对MCF-7、NIH 3T3和CT-26细胞系进行细胞毒性评估。分别通过β-半乳糖苷酶测定和荧光分光光度法观察并定量MCF-7细胞中FITC标记DNA的最大释放转染率和时间进程。所报道的结果表明，所研究的嵌段共聚物可能有助于增强聚阳离子介导的转染，并可在基因治疗中得到应用。
