Banchet V, Michel J, Jallot E, Wortham L, Bouthors S, Laurent-Maquin D, Balossier G
Laboratoire de Tribologie et Dynamique des Systèmes, UMR CNRS 5513, Ecole Centrale de Lyon, 36 Av Guy de Collongue, 69134 Ecully Cedex, France.
Acta Biomater. 2006 May;2(3):349-59. doi: 10.1016/j.actbio.2006.01.005. Epub 2006 Mar 24.
Short-term physico-chemical reactions at the interface between bioactive glass particles and biological fluids are studied for three glasses with different bioactive properties; these glasses are in the SiO(2)-Na(2)O-CaO-P(2)O(5)-K(2)O-Al(2)O(3)-MgO system. Our aim is to show the difference between the mechanisms of their surface reactions. The relation between the composition and the bioactive properties of these glasses is also discussed. The elemental analysis is performed at the submicrometer scale by scanning transmission electron microscopy associated with energy-dispersive X-ray spectroscopy and electron energy loss spectroscopy. After different immersion times (ranging from 0 to 96 h) of bioactive glass particles in a simulated biological solution, results show the formation of different surface layers at the glass periphery in the case of two bioactive glasses (A9 and BVA). For the third glass (BVH) we do not observe any surface layer formation or any modification of the glass composition. For the two other glasses (A9 and BVA), we observe the presence of different layers: an already observed (Si, O, Al) rich layer at the periphery, a previously demonstrated thin (Si, O) layer formed on top of the (Si, O, Al) layer and a (Ca, P) layer. We determine the different steps of the mechanisms of the surface reactions, which appear to be similar in these glasses, and compare the physico-chemical reactions and kinetics using the different immersion times. The A9 glass permits the observation of all important steps of the surface reactions which lead to bioactivity. This study shows the important relationship between composition and bioactivity which can determine the medical applicability of the glass.
研究了具有不同生物活性的三种玻璃在生物活性玻璃颗粒与生物流体界面处的短期物理化学反应；这些玻璃属于SiO(2)-Na(2)O-CaO-P(2)O(5)-K(2)O-Al(2)O(3)-MgO体系。我们的目的是展示它们表面反应机制之间的差异。还讨论了这些玻璃的组成与生物活性之间的关系。通过与能量色散X射线光谱和电子能量损失光谱相关联的扫描透射电子显微镜在亚微米尺度上进行元素分析。将生物活性玻璃颗粒在模拟生物溶液中浸泡不同时间（0至96小时）后，结果表明，对于两种生物活性玻璃（A9和BVA），在玻璃周边形成了不同的表面层。对于第三种玻璃（BVH），我们未观察到任何表面层形成或玻璃组成的任何变化。对于另外两种玻璃（A9和BVA），我们观察到存在不同的层：周边已观察到的富含（Si、O、Al）的层、在（Si、O、Al）层顶部形成的先前已证明的薄（Si、O）层以及（Ca、P）层。我们确定了表面反应机制的不同步骤，这些步骤在这些玻璃中似乎相似，并使用不同的浸泡时间比较物理化学反应和动力学。A9玻璃能够观察到导致生物活性的表面反应的所有重要步骤。这项研究表明了组成与生物活性之间的重要关系，这可以决定玻璃的医学适用性。
