Lee Jong Hyouk, Kang Hee Joon, Woo Jeong-Soo, Chae Sung Won, Lee Sang Hag, Hwang Soon Jae, Lee Heung-Man
Department of Otorhinolaryngology-Head and Neck Surgery, Hallym University College of Medicine, Seoul, South Korea.
Arch Otolaryngol Head Neck Surg. 2006 May;132(5):537-41. doi: 10.1001/archotol.132.5.537.
To investigate the up-regulation of chemokine ligand 20 (CCL20) in chronic rhinosinusitis mucosa and to localize the distribution of CCL20 in the human paranasal sinus mucosa.
Prospective study.
Tertiary academic institution.
Ten patients who underwent functional endoscopic sinus surgery for chronic rhinosinusitis without nasal polyps and 10 normal control subjects.
Messenger RNA was extracted from the sinus mucosa, and semiquantitative reverse transcriptase-polymerase chain reaction was performed. Immunohistochemical staining was used to localize the CCL20 protein.
The expression levels of CCL20 messenger RNA level in chronic rhinosinusitis without nasal polyps were significantly increased compared with those in normal sinus mucosa. The expression of CCL20 protein was greater in chronic rhinosinusitis without nasal polyps mucosa and was localized to the epithelial and submucosal glandular cells.
CCL20 is an inducible product of human paranasal sinus epithelium that may play a role in modulating mucosal immunity of the sinus mucosa.
研究趋化因子配体20（CCL20）在慢性鼻-鼻窦炎黏膜中的上调情况，并确定CCL20在人鼻窦黏膜中的分布位置。
前瞻性研究。
三级学术机构。
10例因慢性鼻-鼻窦炎且无鼻息肉而接受功能性鼻内镜鼻窦手术的患者以及10名正常对照者。
从鼻窦黏膜中提取信使核糖核酸，并进行半定量逆转录-聚合酶链反应。采用免疫组织化学染色法确定CCL20蛋白的位置。
与正常鼻窦黏膜相比，无鼻息肉的慢性鼻-鼻窦炎患者CCL20信使核糖核酸水平的表达显著增加。无鼻息肉的慢性鼻-鼻窦炎黏膜中CCL20蛋白的表达更高，且定位于上皮细胞和黏膜下腺细胞。
CCL20是人类鼻窦上皮的一种诱导产物，可能在调节鼻窦黏膜的黏膜免疫中发挥作用。
