Laupu W, Brimacombe J, Richards E, Keller C
Department of Anaesthesia and Intensive Care, Cairns Base Hospital, Australia.
Anaesthesia. 2006 Jun;61(6):524-7. doi: 10.1111/j.1365-2044.2006.04642.x.
In this three-stage study, we test the hypothesis that supplementary cleaning with potassium permanganate > or =4 mg.l(-1) eliminates protein and particle contamination from the reusable Classic laryngeal mask airway. The first stage involved supplementary cleaning of 70 1 x 1 cm segments from deliberately contaminated laryngeal mask airways using potassium permanganate at 0, 2, 4, 8, 16, 32 and 64 mg.l(-1) and testing for protein staining. This showed that the lowest concentration required to eliminate protein contamination was 8 mg.l(-1). The second stage involved supplementary cleaning of 50 used laryngeal mask airways with either potassium permanganate 8 mg.l(-1) or saline and testing for protein staining. This showed that protein contamination was lower in the potassium permanganate group (p < 0.00001): all laryngeal mask airways in the control group and none in the potassium permanganate group were contaminated. The third stage involved scanning electron microscopic examination of 1 x 1 cm segments from three laryngeal mask airways used in the control group, three from the potassium permanganate group, plus three brand new laryngeal mask airways. The mean density of > or =1 mum surface particles was lower in the potassium permanganate 8 mg.l(-1) than the control group (21 vs. 121 .cm(-2), p < 0.0001) and was similar to brand new laryngeal mask airways (24 .cm(-2)). We conclude that supplementary cleaning with potassium permanganate 8 mg.l(-1) eliminates protein deposits from reusable laryngeal mask airways and reduces particle contamination to similar levels to brand new laryngeal mask airways.
在这项三阶段研究中，我们检验了以下假设：使用浓度≥4 mg·l⁻¹的高锰酸钾进行补充清洁可消除可重复使用的经典喉罩气道上的蛋白质和颗粒污染物。第一阶段，我们使用浓度分别为0、2、4、8、16、32和64 mg·l⁻¹的高锰酸钾对故意污染的喉罩气道上70个1×1 cm的节段进行补充清洁，并检测蛋白质染色情况。结果表明，消除蛋白质污染所需的最低浓度为8 mg·l⁻¹。第二阶段，我们使用8 mg·l⁻¹的高锰酸钾或生理盐水对50个使用过的喉罩气道进行补充清洁，并检测蛋白质染色情况。结果显示，高锰酸钾组的蛋白质污染程度更低（p < 0.00001）：对照组的所有喉罩气道均被污染，而高锰酸钾组无一被污染。第三阶段，我们对对照组使用的3个喉罩气道、高锰酸钾组使用的3个喉罩气道以及3个全新的喉罩气道上1×1 cm的节段进行扫描电子显微镜检查。8 mg·l⁻¹高锰酸钾组中≥1 µm表面颗粒的平均密度低于对照组（分别为21个/cm²和121个/cm²，p < 0.0001），且与全新喉罩气道相似（24个/cm²）。我们得出结论，使用8 mg·l⁻¹的高锰酸钾进行补充清洁可消除可重复使用喉罩气道上的蛋白质沉积物，并将颗粒污染物减少到与全新喉罩气道相似的水平。
