Darnell Malin, Aras Hülya Cevik, Magnusson B, Ekström J
Department of Pharmacology, Sahlgrenska Academy at Göteborg University, Medicinaregatan 15 D, P.O. Box 431, Göteborg 405 30, Sweden.
Arch Oral Biol. 2006 Sep;51(9):769-74. doi: 10.1016/j.archoralbio.2006.03.013. Epub 2006 May 15.
Antimicrobial beta-defensins are thought to protect epithelial surfaces. Their mobilization in response to inflammation was studied in the rat parotid gland using an ELISA assay. Bacterial lipopolysaccharide (LPS), injected into the parotid duct on one side, induced a marked local inflammatory response in the parotid gland as judged by several fold increases in myeloperoxidase activity and, in histological sections, infiltration of neutrophils. Three hours after the injection, beta-defensin 1 and 3 were increased (by 41% and 15%, respectively, P<0.01) as compared to the contralateral gland. Though still elevated 6h after the injection, the percentage figures for beta-defensin 1 were, at this time, somewhat lower (30%) compared to the situation at 3h, while those for defensin 3 were significantly higher 65% (P<0.01); neither at the early nor at the late time of observation were any changes in the level of beta-defensin 2 observed. The beta-defensins under study were not detected in submandibular and sublingual glands, neither were they detected in the inflamed submandibular gland, showing also here several fold increases in myeloperoxidase activity and, in addition, the presence of inflammatory cells, following ductal injection of LPS towards the gland.
抗菌β-防御素被认为可保护上皮表面。利用酶联免疫吸附测定法（ELISA）在大鼠腮腺中研究了它们对炎症反应的调动情况。向一侧腮腺导管注射细菌脂多糖（LPS）后，通过髓过氧化物酶活性增加数倍以及组织学切片中嗜中性粒细胞浸润判断，腮腺中引发了明显的局部炎症反应。注射后3小时，与对侧腺体相比，β-防御素1和3增加（分别增加41%和15%，P<0.01）。尽管注射后6小时仍升高，但此时β-防御素1的百分比数值比3小时时略低（30%），而防御素3的百分比数值显著更高（65%，P<0.01）；在观察的早期和晚期均未观察到β-防御素2水平有任何变化。在颌下腺和舌下腺中未检测到所研究的β-防御素，在向颌下腺导管注射LPS后，颌下腺出现炎症，髓过氧化物酶活性同样增加数倍，此外还存在炎症细胞，也未检测到β-防御素。
