Lin Yu-Te, Berger Richard A, Berger Evelyn J, Tomita Kazunari, Jew Jean Y, Yang Chao, An Kai-Nan
Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, Minnesota 55905, USA.
J Orthop Res. 2006 Jun;24(6):1225-30. doi: 10.1002/jor.20166.
As part of an investigation of the articular nerve ending populations in the wrist joint capsule associated with the anterior and posterior interosseous nerves, this study addresses the nerve ending population in the dorsal radiocarpal ligament. The ligaments were harvested from four wrists of two fresh cadavers within 12 h of death. Tissues were fixed, cryostat sectioned, and processed for fluorescence immunohistochemistry using antibody to protein gene product 9.5 (PGP 9.5), a general or pan neuronal marker, and a secondary antibody conjugated to a fluorescent tag (Alexa Fluor 488). The sections were evaluated with a confocal laser microscope and an image analyzer. Labeled nerve endings were mapped, measured, and categorized. Type I (Ruffini-like ending), Type III (Golgi-like tendon organ) and Type IV (noncorpuscular) nerve endings could be identified in all four DRC ligaments, with Types I and IV dominating. These receptors were distributed primarily over the superficial two thirds of the ligament (>80%), and near the bony attachments (>70%). The dorsal radiocarpal ligament has a rich sensory innervation from the posterior interosseous nerve terminating in nerve endings located in the superficial two-thirds of the ligaments, primarily near bony attachment sites.
作为对与骨间前神经和骨间后神经相关的腕关节囊内关节神经末梢群体研究的一部分，本研究关注桡腕背侧韧带中的神经末梢群体。韧带取自两具新鲜尸体的四只手腕，死亡时间在12小时内。组织经固定、冷冻切片，并使用针对蛋白基因产物9.5（PGP 9.5，一种通用或泛神经元标志物）的抗体和与荧光标签（Alexa Fluor 488）偶联的二抗进行荧光免疫组织化学处理。切片用共聚焦激光显微镜和图像分析仪进行评估。对标记的神经末梢进行绘图、测量和分类。在所有四条桡腕背侧韧带中均可识别出I型（类鲁菲尼末梢）、III型（类高尔基腱器官）和IV型（非小体性）神经末梢，其中I型和IV型占主导。这些感受器主要分布在韧带的浅表三分之二（>80%）以及靠近骨附着处（>70%）。桡腕背侧韧带拥有来自骨间后神经的丰富感觉神经支配，这些神经末梢终止于韧带浅表三分之二处，主要靠近骨附着部位。
